関連する実験動画
Updated: May 11, 2026

09:42
Immunostaining for DNA Modifications: Computational Analysis of Confocal Images
Published on: September 7, 2017
RNA誘導DNAメチル化部位におけるポリメラーゼIVの占有には,SHH1が必要である
Julie A Law1, Jiamu Du, Christopher J Hale
1Department of Molecular, Cell and Developmental Biology, University of California at Los Angeles, Los Angeles, California 90095, USA.
Nature
|May 3, 2013
まとめ
SAWADEE HOMEODOMAIN HOMOLOG 1 (SHH1) タンパク質は,Arabidopsis.でsiRNAの生成とDNAメチル化ターゲティングを可能にしています. SHH1はSHH1で,SHH1はSHH1で
科学分野:
- エピジェネティクス エピジェネティクス
- 植物分子生物学 植物分子生物学
- 遺伝子規制 遺伝子規制
背景:
- DNAメチル化は,遺伝子サイレンシングとゲノムの安定性にとって極めて重要です.
- アラビドプシスのRNA誘導DNAメチル化 (RdDM) 経路は,小さな干渉RNA (siRNA) と植物特異のRNAポリメラーゼ (Pol-IV,Pol-V) を使用しています.
- Pol-IVのターゲティングメカニズムを理解することは,遺伝子発現を制御するために不可欠です.
研究 の 目的:
- RdDM経路におけるSAWADEE HOMEODOMAIN HOMOLOG 1 (SHH1) の機能を調査する.
- Pol-IVターゲティングのメカニズムとその規制を解明する.
- SHH1の染色体結合モジュールの役割を調査する.
主な方法:
- アラビドプシスのSHH1機能を研究した.
- RdDMターゲットのsiRNA生成とPol-IV占有率を分析した.
- SHH1 SAWADEEドメインの染色素結合特性を特徴づけた.
- 重要なSHH1残留物のインビボ機能を評価した.
主要な成果:
- SHH1は,RdDM経路の上流で作用し,siRNAの産生と,RdDMの活性ターゲットのPol-IV占拠を可能にします.
- SHH1 SAWADEEドメインは,未メチル化H3K4およびメチル化H3K9.9を認識する新しい二重リジンリーダです.
- SHH1のライシン結合ポケットの重要な残留物は,siRNAレベル,DNAメチル化,Pol-IV占有率を維持するために不可欠です.
結論:
- SHH1は,RdDM経路内の特定の位置にPol-IVを標的にする上で重要な役割を果たします.
- SHH1 SAWADEEドメインの二重リンシン結合活動は,その機能の中心にある.
- SHH1のメカニズムの理解は,農業と遺伝子治療に関連した表遺伝子制御の洞察を提供します.
関連する概念動画
Restarting Stalled Replication Forks
DNA replication is initiated at sites containing predefined DNA sequences known as origins of replication. DNA is unwound at these sites by the minichromosome maintenance (MCM) helicase and other factors such as Cdc45 and the associated GINS complex.The unwound single strands are protected by replication protein A (RPA) until DNA polymerase starts synthesizing DNA at the 5’ end of the strand in the same direction as the replication fork. To prevent the replication fork from falling apart, a...
Eukaryotic RNA Polymerases
RNA Polymerase (RNAP) is conserved in all animals, with bacterial, archaeal, and eukaryotic RNAPs sharing significant sequence, structural, and functional similarities. Among the three eukaryotic RNAPs, RNA Polymerase II is most similar to bacterial RNAP in terms of both structural organization and folding topologies of the enzyme subunits. However, these similarities are not reflected in their mechanism of action.
All three eukaryotic RNAPs require specific transcription factors, of which the...
All three eukaryotic RNAPs require specific transcription factors, of which the...
Eukaryotic RNA Polymerases
RNA Polymerase (RNAP) is conserved in all animals, with bacterial, archaeal, and eukaryotic RNAPs sharing significant sequence, structural, and functional similarities. Among the three eukaryotic RNAPs, RNA Polymerase II is most similar to bacterial RNAP in terms of both structural organization and folding topologies of the enzyme subunits. However, these similarities are not reflected in their mechanism of action.
All three eukaryotic RNAPs require specific transcription factors, of which the...
All three eukaryotic RNAPs require specific transcription factors, of which the...
Transcription Initiation
Initiation is the first step of transcription in eukaryotes. Prokaryotic RNA Polymerase (RNAP) can bind to the template DNA and start transcribing. On the other hand, transcription in eukaryotes requires additional proteins, called transcription factors, to first bind to the promoter region in the DNA template. This binding helps recruit the specific RNAP that can assemble on the DNA and start transcription.
The promoters and enhancers and their accessory proteins allow tight regulation of...
The promoters and enhancers and their accessory proteins allow tight regulation of...
Translesion DNA Polymerases
Translesion (TLS) polymerases rescue stalled DNA polymerases at sites of damaged bases by replacing the replicative polymerase and installing a nucleotide across the damaged site. Doing so, TLS allows additional time for the cell to repair the damage before resuming regular DNA replication.
TLS polymerases are found in all three domains of life - archaea, bacteria, and eukaryotes. Of the different classes of TLS polymerases, members of the Y family are fitted with specialized structures that...
TLS polymerases are found in all three domains of life - archaea, bacteria, and eukaryotes. Of the different classes of TLS polymerases, members of the Y family are fitted with specialized structures that...
RNA Polymerase II Accessory Proteins
Proteins that regulate transcription can do so either via direct contact with RNA Polymerase or through indirect interactions facilitated by adaptors, mediators, histone-modifying proteins, and nucleosome remodelers. Direct interactions to activate transcription is seen in bacteria as well as in some eukaryotic genes. In these cases, upstream activation sequences are adjacent to the promoters, and the activator proteins interact directly with the transcriptional machinery. For example, in...
