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関連する概念動画

RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
Cell Specific Gene Expression01:58

Cell Specific Gene Expression

Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
Cell Specific Gene Expression01:58

Cell Specific Gene Expression

Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...

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Updated: May 11, 2026

Single-cell Gene Expression Using Multiplex RT-qPCR to Characterize Heterogeneity of Rare Lymphoid Populations
10:23

Single-cell Gene Expression Using Multiplex RT-qPCR to Characterize Heterogeneity of Rare Lymphoid Populations

Published on: January 19, 2017

単細胞トランスクリプトミックは,免疫細胞の発現とスプライシングにおけるバイモダリティを明らかにする.

Alex K Shalek1, Rahul Satija, Xian Adiconis

  • 1Department of Chemistry and Chemical Biology, Harvard University, 12 Oxford Street, Cambridge, Massachusetts 02138, USA.

Nature
|May 21, 2013
PubMed
まとめ

個々の細胞は,重要な遺伝子発現の違いを示しています. 単細胞RNA配列解析は,マウスのデンドリット細胞における免疫遺伝子発現とスプライシングパターンの広範なバイモダル変異を明らかにしています.

さらに関連する動画

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations
09:34

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations

Published on: October 25, 2018

関連する実験動画

Last Updated: May 11, 2026

Single-cell Gene Expression Using Multiplex RT-qPCR to Characterize Heterogeneity of Rare Lymphoid Populations
10:23

Single-cell Gene Expression Using Multiplex RT-qPCR to Characterize Heterogeneity of Rare Lymphoid Populations

Published on: January 19, 2017

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations
09:34

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations

Published on: October 25, 2018

科学分野:

  • 免疫学 免疫学とは
  • ゲノミクスゲノミクスとは
  • 細胞生物学 細胞生物学

背景:

  • 集団内の個々の細胞は,有意な分子および機能的多様性を示す.
  • 以前の研究は,単細胞の複数の分子を同時に測定できないことによって制限されていました.
  • 単細胞分析のためのゲノムプロファイリング方法が現在利用可能になっています.

研究 の 目的:

  • 単細胞RNA配列解析を用いてマウスの骨髄由来 dendritic cells (BMDCs) の細胞異質性を調査する.
  • 遺伝子発現とスプライシングパターンのこれまで観察されなかった変異を特定する.
  • 細胞の多様性を支える規制回路を理解する.

主な方法:

  • マウスの骨髄由来デンドリット細胞 (BMDCs) の単細胞RNA配列解析 (scRNA-seq) について.
  • 選択されたトランスクリプトの検証のためのRNA-光 in situ ハイブリダイゼーション (FISH).
  • 個々の細胞における遺伝子発現とスプライシングパターンの分析.
  • 制御回路を調査するためにノックアウトマウスモデルを使用.

主要な成果:

  • メッセンジャーRNAの豊富性とスプライシングパターンの広範な,これまで観察されなかったバイモダル変異が特定されました.
  • 何百もの重要な免疫遺伝子がバイモダルの発現を示し,非常に発現している遺伝子でさえも.
  • スプライシングパターンは,個々の細胞間の有意な異質性を明らかにした.
  • 137の共同調節された抗ウイルス反応遺伝子のモジュールが特定されました.
  • このモジュールの変動は,Stat2とIrf7.7を含むインターフェロンフィードバック回路と関連していました.

結論:

  • 単細胞ゲノミクスは,機能的多様性を強力に明らかにし,細胞の状態と回路を明らかにします.
  • バイモダル遺伝子発現とスプライシングは,BMDCにおける細胞の異質性に寄与する.
  • インターフェロンフィードバック回路は,遺伝子発現の変動性を広げる役割を果たします.