Jove
Visualize
お問い合わせ
JoVE
x logofacebook logolinkedin logoyoutube logo
JoVEについて
概要リーダーシップブログJoVEヘルプセンター
著者向け
出版プロセス編集委員会範囲と方針査読よくある質問投稿
図書館員向け
推薦の声購読アクセスリソース図書館諮問委員会よくある質問
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experimentsアーカイブ
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教員リソースセンター教員サイト
利用規約
プライバシーポリシー
ポリシー

関連する概念動画

The Extracellular Matrix01:42

The Extracellular Matrix

Overview
The Extracellular Matrix01:29

The Extracellular Matrix

Overview
In order to maintain tissue organization, many animal cells are surrounded by structural molecules that make up the extracellular matrix (ECM). Together, the molecules in the ECM maintain the structural integrity of tissue as well as the remarkable specific properties of certain tissues.
Composition of the Extracellular Matrix
The extracellular matrix (ECM) is commonly composed of ground substance, a gel-like fluid, fibrous components, and many structurally and functionally diverse...
Extracellular Matrix01:26

Extracellular Matrix

Unlike epithelial tissue, which is composed of cells closely packed with little or no extracellular space in between, connective tissue cells are dispersed in a matrix. This extracellular matrix (ECM) is composed of fibrous proteins like collagen, elastin, and fibronectin in a ground substance consisting of interstitial fluid, cell adhesion proteins, and proteoglycans. The proteoglycans form a gel-like material in the spaces between cells and provide hydration, buffering, binding, and force...
Role of Matrix Metalloproteases in Degradation of ECM01:23

Role of Matrix Metalloproteases in Degradation of ECM

Matrix metalloproteases (MMPs) are enzymes involved in the hydrolysis of proteins and glycoproteins of the extracellular matrix. MMPs are essential for the migration and proliferation of cells through the dense matrix network, throughout embryonic development, and throughout morphogenesis. The first MMP activity discovered was a collagenase in a tadpole's tail undergoing metamorphosis. The active collagen deposition and modifications lead to the morphogenesis of tadpoles into the adult body.
A...
Overview of Cell-Matrix Interactions01:24

Overview of Cell-Matrix Interactions

The extracellular matrix or ECM holds cells together to form a tissue and allows the cells within the tissue to communicate. ECM comprises proteins such as fibronectin, collagen, laminin, etc. The most abundant protein in this space is collagen. Collagen fibers are interwoven with carbohydrate-containing protein molecules called proteoglycans. ECM allows cell migration and provides a structural scaffold at cell adhesion that anchors the cell when the extracellular matrix proteins interact with...
Cell-matrix's Response to Mechanical Forces01:13

Cell-matrix's Response to Mechanical Forces

In animal cells, the extracellular matrix allows cells within tissues to withstand external stresses and transmits signals from the outside of the cell to the inside. The extracellular matrix is extensive, and its composition varies between different types of tissues. For example, the reticular fibers and ground substance make up the ECM in loose connective tissue, while collagen and bone minerals make up the ECM of bone tissue. 
Anchoring junctions mechanically attach a cell to the...

こちらも読む

関連記事

共著者、ジャーナル、引用グラフによってこの研究に関連する記事。

並び替え
Same author

Cryo-electron microscopy of mucins.

Methods in enzymology·2026
Same author

Spatial Relations between Coccoliths and Their Confining Membrane During Crystal Morphogenesis.

Journal of the American Chemical Society·2026
Same author

Bridging nanomechanics and bioenergetics of single mitochondria by atomic force microscopy.

Life science alliance·2026
Same author

Elucidating Structural Disorder in a Polymeric Layered Material: The Case of Sodium Poly(heptazine imide) Photocatalyst.

Nano letters·2025
Same author

Young secretory proteins go through a phase.

Nature cell biology·2025
Same author

Protein Target Highlights in CASP16: Insights From the Structure Providers.

Proteins·2025

関連する実験動画

Updated: May 11, 2026

Using Cell-substrate Impedance and Live Cell Imaging to Measure Real-time Changes in Cellular Adhesion and De-adhesion Induced by Matrix Modification
09:11

Using Cell-substrate Impedance and Live Cell Imaging to Measure Real-time Changes in Cellular Adhesion and De-adhesion Induced by Matrix Modification

Published on: February 19, 2015

分泌されるジスルファイド触媒は,細胞外マトリックス組成と機能を制御する.

Tal Ilani1, Assaf Alon, Iris Grossman

  • 1Department of Structural Biology, Weizmann Institute of Science, Rehovot, Israel. tal.ilani@weizmann.ac.il

Science (New York, N.Y.)
|May 25, 2013
PubMed
まとめ
この要約は機械生成です。

クエスチン硫ヒドリル酸化酵素1 (QSOX1) は,ラミニンを細胞外マトリックス (ECM) に組み込むのに不可欠です. QSOX1がなければ,ECMは欠陥があり,細胞の結合と移動を損なう.

さらに関連する動画

Combining Non-reducing SDS-PAGE Analysis and Chemical Crosslinking to Detect Multimeric Complexes Stabilized by Disulfide Linkages in Mammalian Cells in Culture
09:37

Combining Non-reducing SDS-PAGE Analysis and Chemical Crosslinking to Detect Multimeric Complexes Stabilized by Disulfide Linkages in Mammalian Cells in Culture

Published on: May 2, 2019

A Rapid, Scalable Method for the Isolation, Functional Study, and Analysis of Cell-derived Extracellular Matrix
09:40

A Rapid, Scalable Method for the Isolation, Functional Study, and Analysis of Cell-derived Extracellular Matrix

Published on: January 4, 2017

関連する実験動画

Last Updated: May 11, 2026

Using Cell-substrate Impedance and Live Cell Imaging to Measure Real-time Changes in Cellular Adhesion and De-adhesion Induced by Matrix Modification
09:11

Using Cell-substrate Impedance and Live Cell Imaging to Measure Real-time Changes in Cellular Adhesion and De-adhesion Induced by Matrix Modification

Published on: February 19, 2015

Combining Non-reducing SDS-PAGE Analysis and Chemical Crosslinking to Detect Multimeric Complexes Stabilized by Disulfide Linkages in Mammalian Cells in Culture
09:37

Combining Non-reducing SDS-PAGE Analysis and Chemical Crosslinking to Detect Multimeric Complexes Stabilized by Disulfide Linkages in Mammalian Cells in Culture

Published on: May 2, 2019

A Rapid, Scalable Method for the Isolation, Functional Study, and Analysis of Cell-derived Extracellular Matrix
09:40

A Rapid, Scalable Method for the Isolation, Functional Study, and Analysis of Cell-derived Extracellular Matrix

Published on: January 4, 2017

科学分野:

  • バイオケミストリー バイオケミストリー
  • 細胞生物学 細胞生物学
  • 細胞外マトリックス生物学 細胞外マトリックス生物学

背景:

  • 分泌タンパク質におけるディスルファイド結合形成は,通常,エンドプラズマ網膜 (ER) で発生する.
  • クエスチン硫ヒドリル酸化酵素1 (QSOX1) は,ゴルギ装置または分泌物で発見される珍しい二硫化触媒です.
  • QSOX1の細胞外機能は,ほとんど特徴づけられていない.

研究 の 目的:

  • 細胞外キエスチン硫ヒドリル酸化酵素1 (QSOX1) が,デノボ・ディスルファイド結合形成における生理学的役割を調査する.
  • 細胞外マトリックス (ECM) の構成と機能に対するQSOX1の影響を決定する.
  • QSOX1阻害が,粘着や移動などの細胞プロセスに及ぼす影響を評価する.

主な方法:

  • 細胞外環境におけるQSOX1の活性を測定するために生化学的測定を用いた.
  • QSOX1機能を阻害するモノクローナル抗体を生成し,使用した.
  • QSOX1活性とQSOX1活性のない線維芽細胞によって合成される細胞外マトリックス (ECM) の組成を分析した.
  • 機能的測定を用いて,細胞-マトリックス結合と細胞移動を評価した.

主要な成果:

  • QSOX1の活動は,フィブロブラストで合成されたECMにラミニンが適切に組み込まれるために不可欠でした.
  • QSOX1活動が欠けているECMは,細胞-マトリックス粘着をサポートする欠陥を示した.
  • モノクローナル抗体を用いたQSOX1の抑制により,ECMの性質が変化し,細胞移動が減少した.

結論:

  • 細胞外QSOX1は,ECMの組立と完全性において重要な役割を果たします.
  • QSOX1媒介による二硫化結合形成は,ECM依存の細胞結合と移動に不可欠である.
  • QSOX1をターゲットにすることは,ECM特性と細胞行動を調節するための潜在的な戦略を提供します.