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関連する概念動画

Lineage Commitment01:21

Lineage Commitment

Commitment is the  process whereby stem cells:
Somatic to iPS Cell Reprogramming01:29

Somatic to iPS Cell Reprogramming

Reprogramming alters the gene expression in somatic cells, transforming them into induced pluripotent stem (iPS) cells over several generations. Scientists can reprogram cells by introducing genes for four transcription factors—Oct4, Sox2, Klf4, and c-Myc (OSKM) by viral or non-viral methods. These factors are also known as Yamanaka factors after Shinya Yamanaka, who first generated iPS cells using mouse skin cells. Yamanaka was awarded the Nobel Prize in Physiology or Medicine in 2012 for this...
Methods of Nuclear Reprogramming01:24

Methods of Nuclear Reprogramming

Nuclear reprogramming is a process of transforming one cell type into an unrelated cell type by epigenetic changes that alter the cell’s original gene expression pattern. Such epigenetic changes force cells to express a different set of genes, which play a significant role in inducing transformation into other cell types. Nuclear reprogramming offers applications in reproductive cloning for livestock propagation and regenerative medicine — developing patient-specific cells for injury repair.
Introduction to Nuclear Reprogramming01:14

Introduction to Nuclear Reprogramming

Nuclear reprogramming is the process of switching gene expression of one cell type to that of another cell type, usually from a differentiated cell state to an undifferentiated cell state. Differentiation occurs during processes such as development and morphogenesis, tissue regeneration, and malignancy. Cells can also be artificially induced to reprogram their gene expression by techniques such as nuclear transfer, induced pluripotency, and cell fusion. Such techniques have many applications in...
Maintenance of the ES Cell State01:14

Maintenance of the ES Cell State

The cells of the blastocyst inner cell mass only remain pluripotent for a short time. This state of pluripotency and self-renewal can be maintained in embryonic stem (ES) cell culture by adding specific chemicals or growth factors to ensure the cells can continue dividing and later differentiate into different cell types. In some cases, the cells are grown on a feeder layer of differentiated cells, which provides the growth factors and extracellular matrix components necessary for stem cell...
Chromatin Modification in iPS Cells01:32

Chromatin Modification in iPS Cells

Chromatin modification alters gene expression; therefore, scientists can add histone-modifying enzymes, histone variants, and chromatin remodeling complexes to somatic cells to aid reprogramming into pluripotent stem (iPS) cells.
Compact chromatin makes reprogramming difficult. Enzymes, such as histone demethylases and acetyltransferases, are often added during reprogramming to loosen the chromatin, making the DNA more accessible to transcription factors. Molecules that inhibit histone...

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関連する実験動画

Updated: May 11, 2026

Chemical Reversion of Conventional Human Pluripotent Stem Cells to a Na&#239;ve-like State with Improved Multilineage Differentiation Potency
09:07

Chemical Reversion of Conventional Human Pluripotent Stem Cells to a Naïve-like State with Improved Multilineage Differentiation Potency

Published on: June 10, 2018

多能性の新たなバランス:系統指定器による再プログラミング

Uri Ben-David1, Jonathan Nissenbaum, Nissim Benvenisty

  • 1Stem Cell Unit, Department of Genetics, Silberman Institute of Life Sciences, The Hebrew University, Jerusalem 91904, Israel.

Cell
|May 28, 2013
PubMed
まとめ

ソマティック細胞をプラリポテンツ状態に再プログラムすることは,系統特異剤を使用して達成できます. このアプローチは,多能性が対極の微分信号のバランスを取ることを含むという考えを支持する.

科学分野:

  • 細胞生物学 細胞生物学
  • 発達生物学 発達生物学とは
  • 幹細胞の研究についてです.

背景:

  • 誘発性多能幹細胞 (iPSCs) への体細胞再プログラミングは,通常,転写因子の組み合わせを使用して達成されます.
  • これらの転写因子は,多能性を維持するコア回路の一部である.
  • 多権国家の安定と維持を統制する正確なメカニズムは,まだ調査中です.

研究 の 目的:

  • ソマティック細胞の再プログラミングのための系統特異剤の使用の有効性を調査する.
  • 多能性の文脈における系統指定者の役割を探求する.
  • ダイナミック・バランスとしての多能性のモデルに対するさらなる証拠を提供する.

主な方法:

  • 再プログラミングプロセスで利用された系統指定符.
  • 結果的に再プログラムされた細胞を分析し,多能性マーカーを検出しました.
  • 再プログラム効率と特性を,伝統的な転写因子ベースの方法と比較した.

主要な成果:

  • 系統特異剤を用いた体細胞における多能性の成功誘導.
  • 系統指定者が再プログラミングを推進できることを実証しました.
  • 発見は,系統指定者が差異化力のバランスに影響することを示唆しています.

さらに関連する動画

Reprogramming Primary Amniotic Fluid and Membrane Cells to Pluripotency in Xeno-free Conditions
09:34

Reprogramming Primary Amniotic Fluid and Membrane Cells to Pluripotency in Xeno-free Conditions

Published on: November 27, 2017

関連する実験動画

Last Updated: May 11, 2026

Chemical Reversion of Conventional Human Pluripotent Stem Cells to a Na&#239;ve-like State with Improved Multilineage Differentiation Potency
09:07

Chemical Reversion of Conventional Human Pluripotent Stem Cells to a Naïve-like State with Improved Multilineage Differentiation Potency

Published on: June 10, 2018

Reprogramming Primary Amniotic Fluid and Membrane Cells to Pluripotency in Xeno-free Conditions
09:34

Reprogramming Primary Amniotic Fluid and Membrane Cells to Pluripotency in Xeno-free Conditions

Published on: November 27, 2017

結論:

  • 系統指定者は,体細胞に多能性を誘発するための効果的なツールです.
  • 多能状態は,微妙な均衡によって,微分化経路の間の微妙な均衡によって維持される.
  • この研究は,細胞の運命と再プログラムに関する基本的な原理のより深い理解に貢献します.