セントロメアタンパク質 CENP-Cによるセントロメア核分裂体認識のための保存されたメカニズム
Hidenori Kato1, Jiansheng Jiang, Bing-Rui Zhou
1Laboratory of Biochemistry and Molecular Biology, National Cancer Institute, Bethesda, MD 20892, USA.
Science (New York, N.Y.)
|June 1, 2013
まとめ
セントロメアタンパク質C (CENP-C) は,ヒストンの変種CENP-Aを認識し,その尾を結合させ,他のヒストンとドッキングします. このメカニズムは保存され,細胞分裂中のキネトコア組立に不可欠です.
科学分野:
- 細胞生物学 細胞生物学
- 分子生物学は分子生物学である.
- 遺伝学 遺伝学とは
背景:
- セントロメアにおけるキネトコア組成は,ミトーシス中の染色体分離に不可欠である.
- セントロメアタンパク質C (CENP-C) は,ヒストンの変種CENP-Aを認識するキネトコア組立に不可欠です.
研究 の 目的:
- CENP-A核個体のCENP-C認識を制御する分子決定因子を明らかにする.
- セントロメアへのタンパク質の徴募の保存メカニズムを理解する.
主な方法:
- タンパク質とタンパク質の相互作用を定義するための生化学的分析.
- CENP-C と CENP-A の核細胞相互作用の構造分析.
主要な成果:
- CENP-Cは,CENP-Aの尾の内にある水害性の領域に結合する.
- CENP-CはヒストンH2AとH2Bの酸性パッチにもドッキングします.
- 保存されたCENP-Cモチーフは,同じ認識メカニズムを使用しています.
結論:
- セントロメアタンパク質の徴募のための保存されたメカニズムには,CENP-CがCENP-Aに水害性相互作用と核細胞ドッキングを通じて結合することが含まれています.
- これは,乱れたペプチドと容易なドッキングを含む新しいヒストンの認識モードを明らかにします.
関連する概念動画
02:30Histone Variants at the Centromere
Histone variants are the histone proteins with structural and sequence variations. These variants may be regarded as “mutant” forms that replace their canonical histone counterparts in the nucleosomes. Specific post-translational modifications on the histone variants enable further chromatin complexity and regulate tissue-specific gene expression. The most common histone variants are from histone H2A, H2B, and linker histone H1 families. However, several variants of histone H3 variants are also...
02:19The Spindle Assembly Checkpoint
The spindle assembly checkpoint is a molecular surveillance mechanism ensuring the fidelity of chromosome segregation during anaphase. The checkpoint monitors the completion of all the prerequisite steps before chromosome segregation to determine whether the segregation process should proceed or be delayed.
Many proteins function together to control the spindle assembly checkpoint. Mutations affecting these proteins may allow cells to proceed into anaphase prematurely, resulting in the...
Many proteins function together to control the spindle assembly checkpoint. Mutations affecting these proteins may allow cells to proceed into anaphase prematurely, resulting in the...
02:25Centrosome Duplication
The primary microtubule organizing center (MTOC) in animal cells is the centrosome. A centrosome has two cylindrical centrioles at its core. Each centriole consists of nine sets of three microtubules held together by proteins. The centrioles are positioned at right angles to each other and surrounded by a shapeless protein cloud called the pericentriolar matrix, or pericentriolar material (PCM).
To ensure that each daughter cell receives a centrosome after cell division, centrosome duplication...
To ensure that each daughter cell receives a centrosome after cell division, centrosome duplication...
02:25Centrosome Duplication
The primary microtubule organizing center (MTOC) in animal cells is the centrosome. A centrosome has two cylindrical centrioles at its core. Each centriole consists of nine sets of three microtubules held together by proteins. The centrioles are positioned at right angles to each other and surrounded by a shapeless protein cloud called the pericentriolar matrix, or pericentriolar material (PCM).
To ensure that each daughter cell receives a centrosome after cell division, centrosome duplication...
To ensure that each daughter cell receives a centrosome after cell division, centrosome duplication...
01:12The Nucleosome Core Particle
Nucleosomes are the DNA-histone complex, where the DNA strand is wound around the histone core. The histone core is an octamer containing two copies of H2A, H2B, H3, and H4 histone proteins.
Nucleosomes, paradoxically, perform two opposite functions simultaneously. On the one hand, their primary aim is to protect the delicate DNA strands from physical damage and help achieve a higher compaction ratio. On the other hand, they must allow polymerase enzymes to access histone-bound DNA during...
Nucleosomes, paradoxically, perform two opposite functions simultaneously. On the one hand, their primary aim is to protect the delicate DNA strands from physical damage and help achieve a higher compaction ratio. On the other hand, they must allow polymerase enzymes to access histone-bound DNA during...
02:10The Nucleosome Core Particle
Nucleosomes are the DNA-histone complex, where the DNA strand is wound around the histone core. The histone core is an octamer containing two copies of H2A, H2B, H3, and H4 histone proteins.
The paradox
Nucleosomes, paradoxically, perform two opposite functions simultaneously. On the one hand, their main responsibility is to protect the delicate DNA strands from physical damage and help achieve a higher compaction ratio. While on the other hand, they must allow polymerase enzymes to access DNA...
The paradox
Nucleosomes, paradoxically, perform two opposite functions simultaneously. On the one hand, their main responsibility is to protect the delicate DNA strands from physical damage and help achieve a higher compaction ratio. While on the other hand, they must allow polymerase enzymes to access DNA...