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関連する概念動画

Renewal of Intestinal Stem Cells01:23

Renewal of Intestinal Stem Cells

The intestinal epithelial lining rapidly renews every 4 to 5 days. The renewal is facilitated by intestinal stem cells (ISCs) located at the base of the crypt– a gland located at the bottom of each villus. ISCs divide asymmetrically to form new stem cells and progenitor daughter cells. The daughter cells are called transit-amplifying (TA) cells which move upwards along the crypt and either differentiate into absorptive cells– the enterocytes or secretory cells– including the goblet,...
Adult Stem Cells01:33

Adult Stem Cells

Stem cells are undifferentiated cells that divide and produce more stem cells or progenitor cells that differentiate into mature, specialized cell types. All the cells in the body are generated from stem cells in the early embryo, but small populations of stem cells are also present in many adult tissues including the bone marrow, brain, skin, and gut. These adult stem cells typically produce the various cell types found in that tissue—to replace cells that are damaged or to continuously renew...
Role Of Notch Signalling In Intestinal Stem Cell Renewal01:12

Role Of Notch Signalling In Intestinal Stem Cell Renewal

Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
Stem Cell Niche01:26

Stem Cell Niche

The stem cell niche is the dynamic microenvironment where stem cells reside. Inside these niches, the cells may remain undifferentiated, undergo high self-renewal, or become lineage-specific progenitors. Stem cells coexist with other niche cells, such as stromal cells. They also interact closely with the ECM. Cell-cell and cell-matrix communication occur via adhesion molecules or soluble factors that signal the stem cells and determine their fate. Stromal cells also provide survival signals to...
Histology of the Small Intestine01:27

Histology of the Small Intestine

The small intestine exhibits a unique histological structure that significantly enhances its function in digestion and nutrient absorption. These structures include circular folds, villi, and various specialized cells that collectively facilitate the digestion of food.
The intestinal lining features transverse folds called circular folds, each housing fingerlike projections known as intestinal villi. These villi are covered by a layer of simple columnar epithelium, also referred to as...
Role of Ephrin-Eph Signalling in Intestinal Stem Cell Renewal01:22

Role of Ephrin-Eph Signalling in Intestinal Stem Cell Renewal

Erythropoietin-producing hepatocellular carcinoma receptor (Eph) and its ligand, Eph receptor-interacting protein (Ephrin) were first discovered in the human carcinoma cell line, hence the name. Ephrin-Eph interaction guides cells to reach their appropriate location in adult tissues. They also play an essential role in the immune system by helping in immune cell migration, adhesion, and activation. Based on their structure and function, Eph is divided into two classes — EphA and EphB.

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関連する実験動画

Updated: May 9, 2026

Protocols for Analyzing the Role of Paneth Cells in Regenerating the Murine Intestine using Conditional Cre-lox Mouse Models
07:48

Protocols for Analyzing the Role of Paneth Cells in Regenerating the Murine Intestine using Conditional Cre-lox Mouse Models

Published on: November 21, 2015

腸内クリプトは,原型幹細胞のコンパートメントである.

Hans Clevers1

  • 1Hubrecht Institute, Royal Netherlands Academy of Arts and Sciences, 3584 CT Utrecht, the Netherlands. h.clevers@hubrecht.eu

Cell
|July 23, 2013
PubMed
まとめ
この要約は機械生成です。

腸内皮質は,成人の幹細胞の研究の重要なモデルとして機能しています. トランスジェニックマウスを用いた研究では,腸の幹細胞における新たな自己再生プロセスが明らかにされ,従来の幹細胞の定義に異議を唱えている.

さらに関連する動画

Improved Swiss-rolling Technique for Intestinal Tissue Preparation for Immunohistochemical and Immunofluorescent Analyses
07:42

Improved Swiss-rolling Technique for Intestinal Tissue Preparation for Immunohistochemical and Immunofluorescent Analyses

Published on: July 13, 2016

Three-Dimensional Culture of Murine Colonic Crypts to Study Intestinal Stem Cell Function Ex Vivo
07:46

Three-Dimensional Culture of Murine Colonic Crypts to Study Intestinal Stem Cell Function Ex Vivo

Published on: October 11, 2022

関連する実験動画

Last Updated: May 9, 2026

Protocols for Analyzing the Role of Paneth Cells in Regenerating the Murine Intestine using Conditional Cre-lox Mouse Models
07:48

Protocols for Analyzing the Role of Paneth Cells in Regenerating the Murine Intestine using Conditional Cre-lox Mouse Models

Published on: November 21, 2015

Improved Swiss-rolling Technique for Intestinal Tissue Preparation for Immunohistochemical and Immunofluorescent Analyses
07:42

Improved Swiss-rolling Technique for Intestinal Tissue Preparation for Immunohistochemical and Immunofluorescent Analyses

Published on: July 13, 2016

Three-Dimensional Culture of Murine Colonic Crypts to Study Intestinal Stem Cell Function Ex Vivo
07:46

Three-Dimensional Culture of Murine Colonic Crypts to Study Intestinal Stem Cell Function Ex Vivo

Published on: October 11, 2022

科学分野:

  • 幹細胞生物学 幹細胞生物学とは
  • 胃腸内科の研究について
  • 再生医学は再生医学である.

背景:

  • 腸内表皮は,急速な自己更新を伴うダイナミックな組織である.
  • 腸内の成人の幹細胞は,組織ホメオスタシスと修復に不可欠です.
  • 腸の幹細胞の行動を理解することは,胃腸疾患の治療に不可欠です.

研究 の 目的:

  • 腸の幹細胞の自己再生運動と構造を研究する.
  • 先進的なマウスモデルを活体内幹細胞追跡に活用する.
  • 古典的な定義から逸脱する新しい幹細胞の行動を探求する.

主な方法:

  • in vivo視覚化のためのトランスジェニックマウスモデルを使用.
  • 幹細胞の子孫を追跡するために,遺伝的系統の追跡を活用する.
  • 分子分析のために,光でマークされた幹細胞を分離する.
  • 培養幹細胞をインビトロで培養して"ミニ腸"を生成する.

主要な成果:

  • 腸の幹細胞で強烈な自己再生運動を示した.
  • 個々の幹細胞とその子孫を in vivo で視覚化し,追跡した.
  • 腸の幹細胞を成功裏に培養し,自己再生する"ミニ腸"を in vitro で作りました.
  • 古典的な幹細胞パラダイムに異議を唱える幹細胞の行動を特定した.

結論:

  • 腸内表皮は,成人の幹細胞の動態を研究するための強力なモデルを提供します.
  • トランスジェニックマウスモデルは,幹細胞の行動に関する前例のない洞察を提供します.
  • 腸の幹細胞の自己再生は,古典的な幹細胞の定義を無視するなど,ユニークな特徴を示しています.