Jove
Visualize
お問い合わせ
JoVE
x logofacebook logolinkedin logoyoutube logo
JoVEについて
概要リーダーシップブログJoVEヘルプセンター
著者向け
出版プロセス編集委員会範囲と方針査読よくある質問投稿
図書館員向け
推薦の声購読アクセスリソース図書館諮問委員会よくある質問
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experimentsアーカイブ
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教員リソースセンター教員サイト
利用規約
プライバシーポリシー
ポリシー

関連する概念動画

Anchoring Junctions01:03

Anchoring Junctions

Anchoring junctions are multiprotein complexes that help cells connect to other cells and the extracellular matrix. Anchoring junctions are present on the lateral and basal surfaces of cells, providing strong and flexible connections. Focal adhesions are often formed due to cell interactions with the ECM substrata, which initiate signal transduction via kinase cascades and other mechanisms. Together, they provide stability and tissue integrity. There are three types of anchoring junctions:...
Overview of Cell-Cell Junctions01:14

Overview of Cell-Cell Junctions

The complex three-dimensional arrangement of cells in any multicellular organism is defined and maintained by interactions of cells with each other and the extracellular matrix. Cell-cell junctions are specialized structures where the multi-protein complexes on one cell interact with the multi-protein complexes on another  cell. These cell junctions are classified  into three main types based on their function — occluding, anchoring, and gap junctions.
Occluding or Tight Junctions
Tight...
Overview of Cell-Cell Junctions01:14

Overview of Cell-Cell Junctions

The complex three-dimensional arrangement of cells in any multicellular organism is defined and maintained by interactions of cells with each other and the extracellular matrix. Cell-cell junctions are specialized structures where the multi-protein complexes on one cell interact with the multi-protein complexes on another  cell. These cell junctions are classified  into three main types based on their function — occluding, anchoring, and gap junctions.
Occluding or Tight Junctions
Tight...
Tight Junctions01:29

Tight Junctions

Tight junctions are molecular seals between cells that prevent the leaking of fluids, ions, and other small solutes across cavities and compartments in multicellular organisms. They are mainly composed of claudin and occludin transmembrane proteins, and other proteins such as tricellulin and JAM (junctional adhesion molecule). All these proteins are 4-pass transmembrane proteins, except JAM, which is a single-pass transmembrane protein belonging to the immunoglobulin superfamily. The...
Assembly of Complex Microtubule Structures01:32

Assembly of Complex Microtubule Structures

Complex microtubule structures are present in resting cells and in dividing cells. In resting cells, they are responsible for maintaining the cellular architecture, tracks for intracellular transport, positioning of organelles, assembly of cilia and flagella. They mediate the bipolar spindle assembly for chromosomal segregation and positioning of the cell division plate in dividing cells. The formation of microtubule complex structures depends on the cell type, cell stage, and cell function.
Cell-matrix's Response to Mechanical Forces01:13

Cell-matrix's Response to Mechanical Forces

In animal cells, the extracellular matrix allows cells within tissues to withstand external stresses and transmits signals from the outside of the cell to the inside. The extracellular matrix is extensive, and its composition varies between different types of tissues. For example, the reticular fibers and ground substance make up the ECM in loose connective tissue, while collagen and bone minerals make up the ECM of bone tissue. 
Anchoring junctions mechanically attach a cell to the...

こちらも読む

関連記事

共著者、ジャーナル、引用グラフによってこの研究に関連する記事。

並び替え
Same author

Morphological control of gut lumen access shapes enteroendoceine cell function.

The Journal of cell biology·2026
Same author

TS3 Asn347 corticosteroid-binding globulin (CBG) glycoform deficiency is associated with mortality in human septic shock.

Journal of the Endocrine Society·2026
Same author

Deciphering mechanical determinants of morphological evolution.

Cell·2026
Same author

Epithelial cell plasticity in metazoans: Evolutionary insights into roles and mechanisms.

Seminars in cell & developmental biology·2026
Same author

Proteomic analysis of FACS-enriched whole nematocysts from the colonial hydrozoan <i>Hydractinia symbiolongicarpus</i>.

Toxicon: X·2026
Same author

Loss of SPECC1L in cranial neural crest cells results in increased hedgehog signaling and frontonasal dysplasia.

Frontiers in physiology·2026

関連する実験動画

Updated: May 9, 2026

Improved Visualization and Quantitative Analysis of Drug Effects Using Micropatterned Cells
15:41

Improved Visualization and Quantitative Analysis of Drug Effects Using Micropatterned Cells

Published on: December 2, 2010

表面接合は,平面のスパインドルの方向性を指示することによって,組織構造を維持します.

Yu-ichiro Nakajima1, Emily J Meyer, Amanda Kroesen

  • 1Stowers Institute for Medical Research, 1000 East 50th Street, Kansas City, Missouri 64110, USA.

Nature
|July 23, 2013
PubMed
まとめ

適切なミトーシス・スパインドルの整合は,上皮組織の整合性を維持するために極めて重要です. 障害は癌につながる可能性がありますが,ScribbledやDiscs large 1のような交差点タンパク質は,このプロセスを調節するのに役立ちます.

科学分野:

  • 細胞生物学 細胞生物学
  • 発達生物学 発達生物学とは
  • がん研究 がん研究

背景:

  • 皮質細胞の増殖は,組織建築のために平面的スパインドルの整列を必要とします.
  • スピンドルのアライメントの障害は,上皮からメゼンキマへの移行と癌と関連しています.
  • スピンドルの方向を調節するインビボメカニズムは完全に理解されていません.

研究 の 目的:

  • 皮質組織における平面の並べ替えのインビボメカニズムを調査する.
  • ミトスのスパインドル指向と上皮質の整合性の主要な調節体を特定する.

主な方法:

  • ドロソフィラのイメージナルディスクをモデルシステムとして利用した.
  • アクトミオシン皮質と結合タンパク質の役割を調査した (Scribbled, Discs large 1).
  • 細胞のデラミネーション,アポトーシス,腫瘍形成に対するスパインドル不整列の観察された効果.

主要な成果:

  • アクトミオシン皮質と交差点スクリブルド/ディスク大1は,平面スパインドルの調整に不可欠である.
  • 欠陥のあるスパインドルのアライメントは,細胞のデラミネーションとアポトーシスと相関しています.
  • 不整合した細胞におけるアポトーシスの抑制は,基礎腫瘍のような質量形成を促進します.

さらに関連する動画

Characterizing Epithelial Wound Healing In Vivo Using the Cnidarian Model Organism Clytia hemisphaerica
07:47

Characterizing Epithelial Wound Healing In Vivo Using the Cnidarian Model Organism Clytia hemisphaerica

Published on: February 10, 2023

Engineering Three-dimensional Epithelial Tissues Embedded within Extracellular Matrix
08:49

Engineering Three-dimensional Epithelial Tissues Embedded within Extracellular Matrix

Published on: July 10, 2016

関連する実験動画

Last Updated: May 9, 2026

Improved Visualization and Quantitative Analysis of Drug Effects Using Micropatterned Cells
15:41

Improved Visualization and Quantitative Analysis of Drug Effects Using Micropatterned Cells

Published on: December 2, 2010

Characterizing Epithelial Wound Healing In Vivo Using the Cnidarian Model Organism Clytia hemisphaerica
07:47

Characterizing Epithelial Wound Healing In Vivo Using the Cnidarian Model Organism Clytia hemisphaerica

Published on: February 10, 2023

Engineering Three-dimensional Epithelial Tissues Embedded within Extracellular Matrix
08:49

Engineering Three-dimensional Epithelial Tissues Embedded within Extracellular Matrix

Published on: July 10, 2016

結論:

  • 交差点介のスパインドルの整合は,上皮質の整合性を維持するために不可欠です.
  • 皮質の極性および細胞死メカニズムには,固有の腫瘍抑制機能があります.