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グルタミン酸受容体のような遺伝子は,葉から葉への傷のシグナル伝達を媒介する
Seyed A R Mousavi1, Adeline Chauvin, François Pascaud
1Department of Plant Molecular Biology, University of Lausanne, Biophore, CH-1015 Lausanne, Switzerland.
Nature
|August 24, 2013
まとめ
傷ついた植物の葉は,電気信号を使って損傷をシグナルし,防御反応を誘発します. 特定のグルタミン酸受容体のような遺伝子 (GLRs) は,この長距離創傷通信に不可欠です.
科学分野:
- 植物生物学 植物生物学
- 分子生物学は分子生物学である.
- バイオフィジックス 生物物理学
背景:
- 傷ついた葉は,遠距離信号を通じて損傷信号を損傷していない部分に送信します.
- このシグナル伝達経路は,植物防衛機構の重要な調節体であるジャスモネート (jasmonates) の生成を伴う.
研究 の 目的:
- 植物における長距離傷信号の基礎となる生体物理的メカニズムを調査する.
- 創傷信号の伝達と,その後の防御反応に関与する遺伝的成分を特定する.
主な方法:
- アラビドプシス・タリアナ (Arabidopsis thaliana) の葉が傷ついた後の表面潜在変化をマッピングするために,非侵襲的な電極を使用しました.
- ジャスモノイル-イソレウシンの蓄積を測定し,現在の注射に対する反応としてトランスクリプトームの変化を分析した.
- GLUTAMATE RECEPTOR-LIKE (GLR) 遺伝子を中心に34の膜タンパク質変異株をスクリーニングしました.
主要な成果:
- 無傷の葉の傷による膜脱極化は,ジャスモネート信号領域と相関しています.
- 現在の注射は,ジャスモノイル-イソレウシンの蓄積を刺激し,ジャスモネートシグナル伝達に関連する遺伝子発現を変化させた.
- GLR3.2,GLR3.3,GLR3.6遺伝子の変異は,傷口誘発の表面潜在変化を減少させ,glr3.3 glr3.6変異は,遠葉のジャスモネート反応遺伝子発現の減少を示した.
結論:
- この研究は,植物の長距離傷のシグナル伝達を理解するための遺伝的基礎を提供します.
- 臓器間の傷口伝達において重要な役割を果たす植物性グルタミン酸受容体のような遺伝子を特定し,動物のシナプス活性遺伝子に類似しています.
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