関連する実験動画
Updated: May 8, 2026
14:17
Nuclear Transfer into Mouse Oocytes
Published on: November 30, 2006
核波1は,卵細胞における転写の再プログラムと正常な発達のために必要である
Kei Miyamoto1, Marta Teperek, Kosuke Yusa
1Wellcome Trust/Cancer Research UK Gurdon Institute, The Henry Wellcome Building of Cancer and Developmental Biology, Cambridge, UK. k.miyamoto@gurdon.cam.ac.uk
Science (New York, N.Y.)
|August 31, 2013
まとめ
Xenopus Wave1は,体細胞のDNAを再プログラムし,胚の発達中に遺伝子を活性化するために不可欠な核タンパク質です. 欠乏すると発達障害を引き起こし,遺伝子の活性化を阻害する.
科学分野:
- 発達生物学 発達生物学とは
- 分子生物学は分子生物学である.
- 細胞生物学 細胞生物学
背景:
- 卵と卵細胞は,発達に不可欠な精子と体内核を再プログラムします.
- 核アクチンとアクチン結合タンパク質は転写に関与しているが,その役割は不明である.
研究 の 目的:
- Xenopus Wave1の転写再プログラムと胚の発達における役割を調査する.
主な方法:
- 波1は卵細胞の核に定着している.
- Xenopus胚におけるWave1のノックダウン.
- ホックス遺伝子の活性化と発達異常の分析.
- 転写機に結合するWave1を調査する.
主要な成果:
- Xenopus Wave1は卵細胞核に存在し,転写再プログラムに不可欠である.
- Wave1のノックダウンは,異常な発達とHox遺伝子の活性化障害につながる.
- Nuclear Wave1は,活性転写因子とRNAポリメラーゼIIと相互作用する.
結論:
- Wave1は母性再プログラム因子として特定されています.
- Wave1は,発達中の遺伝子活性化において重要な役割を果たします.
関連する概念動画
01:24Methods of Nuclear Reprogramming
Nuclear reprogramming is a process of transforming one cell type into an unrelated cell type by epigenetic changes that alter the cell’s original gene expression pattern. Such epigenetic changes force cells to express a different set of genes, which play a significant role in inducing transformation into other cell types. Nuclear reprogramming offers applications in reproductive cloning for livestock propagation and regenerative medicine — developing patient-specific cells for injury repair.
01:14Introduction to Nuclear Reprogramming
Nuclear reprogramming is the process of switching gene expression of one cell type to that of another cell type, usually from a differentiated cell state to an undifferentiated cell state. Differentiation occurs during processes such as development and morphogenesis, tissue regeneration, and malignancy. Cells can also be artificially induced to reprogram their gene expression by techniques such as nuclear transfer, induced pluripotency, and cell fusion. Such techniques have many applications in...
01:29Somatic to iPS Cell Reprogramming
Reprogramming alters the gene expression in somatic cells, transforming them into induced pluripotent stem (iPS) cells over several generations. Scientists can reprogram cells by introducing genes for four transcription factors—Oct4, Sox2, Klf4, and c-Myc (OSKM) by viral or non-viral methods. These factors are also known as Yamanaka factors after Shinya Yamanaka, who first generated iPS cells using mouse skin cells. Yamanaka was awarded the Nobel Prize in Physiology or Medicine in 2012 for this...
02:54Nucleosome Remodeling
Nucleosomes are the basic units of chromatin compaction. Each nucleosome consists of the DNA bound tightly around a histone core, which makes the DNA inaccessible to DNA binding proteins such as DNA polymerase and RNA polymerase. Hence, the fundamental problem is to ensure access to DNA when appropriate, despite the compact and protective chromatin structure.
Nucleosome remodeling complex
Eukaryotic cells have specialized enzymes called ATP-dependent nucleosome remodeling enzymes. These enzymes...
Nucleosome remodeling complex
Eukaryotic cells have specialized enzymes called ATP-dependent nucleosome remodeling enzymes. These enzymes...
01:17Transcription
Transcription is the synthesis of RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in correctly synthesizing messenger RNA (mRNA). Transcriptional regulation is responsible for the differentiation of different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds of RNA Molecules
In eukaryotes,...
Transcription Can Produce Different Kinds of RNA Molecules
In eukaryotes,...
01:10Transcription
Overview
Transcription is the process of synthesizing RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in the proper synthesis of messenger RNA (mRNA). Regulation of transcription is responsible for the differentiation of all the different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds...
Transcription is the process of synthesizing RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in the proper synthesis of messenger RNA (mRNA). Regulation of transcription is responsible for the differentiation of all the different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds...