関連する実験動画

Updated: May 8, 2026

Efficient Generation of hiPSC Neural Lineage Specific Knockin Reporters Using the CRISPR/Cas9 and Cas9 Double Nickase System
14:46

Efficient Generation of hiPSC Neural Lineage Specific Knockin Reporters Using the CRISPR/Cas9 and Cas9 Double Nickase System

Published on: May 28, 2015

RNA誘導のCRISPR Cas9によるダブルニッキングにより,ゲノム編集の特異性を強化する

F Ann Ran1, Patrick D Hsu, Chie-Yu Lin

  • 1Broad Institute of MIT and Harvard, 7 Cambridge Center, Cambridge, MA 02142, USA; McGovern Institute for Brain Research, Massachusetts Institute of Technology, Cambridge, MA 02139, USA; Department of Brain and Cognitive Sciences, Massachusetts Institute of Technology, Cambridge, MA 02139, USA; Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA; Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138, USA.

Cell
|September 3, 2013
PubMed
まとめ

この研究では,精密なゲノム編集のためのペア化されたCas9ニカゼ法が導入されています. このアプローチは,意図しないDNA変異を大幅に減らし,遺伝子編集アプリケーションの安全性と正確性を高めます.

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Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms09:51

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

Published on: May 25, 2018

Selection-dependent and Independent Generation of CRISPR/Cas9-mediated Gene Knockouts in Mammalian Cells11:35

Selection-dependent and Independent Generation of CRISPR/Cas9-mediated Gene Knockouts in Mammalian Cells

Published on: June 16, 2017

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