関連する実験動画

Updated: Aug 14, 2026

The Production of C. elegans Transgenes via Recombineering with the galK Selectable Marker
12:03

The Production of C. elegans Transgenes via Recombineering with the galK Selectable Marker

Published on: January 12, 2011

酵母GAL4タンパク質とファグT7RNAポリメラーゼのキメラによるテンプレートスーパーコーリング

E A Ostrander1, P Benedetti, J C Wang

  • 1Department of Biochemistry and Molecular Biology, Harvard University, Cambridge, MA 02138.

Science (New York, N.Y.)
|September 14, 1990
PubMed
まとめ

研究者は,酵母 GAL4 DNA結合ドメインと細菌 T7 RNAポリメラーゼを融合させ,キメリック酵素を作り出した. このエンジニアリングされたタンパク質は,特異的に,転写中にT7プロモーター配列を含むDNAをスーパーコイルします.

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