ラミニンA鎖mRNAの一時的および局所的に制限された発現は,腎臓の器官生成中に表皮細胞を発達させることで起こります
M Ekblom1, G Klein, G Mugrauer
1Friedrich-Miescher-Laboratorium, Max-Planck-Gesellschaft, Tübingen, Federal Republic of Germany.
Cell
|January 26, 1990
まとめ
マウスの腎臓の発達には,特定のラミニン (地下膜タンパク質) 鎖が含まれています. ラミニンAの発現は上皮の極化とともに増加し,他のラミニン鎖とは異なり,腎臓の形態変生における一時的な役割を示唆する.
科学分野:
- 発達生物学 発達生物学について
- 細胞生物学 細胞生物学
- バイオケミストリー バイオケミストリー
背景:
- ラミニンは,ポリペプチド鎖からなる重要な基礎膜タンパク質です.
- マウスラミニンは,A,B1,B2の3つのポリペプチド鎖で記述されています.
研究 の 目的:
- ネズミの腎臓発達中のラミニンA,B1,B2mRNAの発現パターンを調査する.
- 腎臓の形態変異の間に特定の細胞の局所とラミニンAの時間的発現を決定する.
主な方法:
- 発育中のマウスの腎臓におけるラミニンA,B1,B2mRNAの発現を,in vitro微分化モデルを用いて研究した.
- 分析されたラミニンA mRNAとポリペプチド発現 in vivo 腎臓発達の過程.
主要な成果:
- ラミニンB1およびB2mRNA発現は,インビトロ腎臓メゼンキーム分化1日目に増加しました.
- ラミニンAmRNA発現は2日目に増加し,上皮細胞の偏極化の開始と一致しました.
- In vivoでは,ラミニンA mRNAとポリペプチドはエピテリアによってのみ発現し,一部の偏光細胞にはA鎖が欠けていた.
結論:
- ラミニンAは,腎臓形態変異の特定の部位に関連した一時的な形態です.
- ラミニンのイソフォームは,A鎖が欠けているか,または異なるA鎖を有するが,より広範な分布パターンを表している.
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