FUS3は,ミトーシスから結合への移行に必要なcdc2+/cdc28関連キナーゼをコードする

E A Elion¹, P L Grisafi, G R Fink

¹Whitehead Institute for Biomedical Research, Nine Cambridge Center, Massachusetts 02142.

Cell

|February 23, 1990

まとめ

FUS3遺伝子は,酵母細胞サイクル停止と交配に不可欠です. Fus3変異体は停止することができませんが,この欠陥はCLN3の変異によって抑制され,FUS3がFUS3であることを示しています.

科学分野:

* 分子・細胞生物学
* イースト・ジェネティクス
* 細胞サイクル規制について

背景:

* 細胞サイクル進行と交配は,酵母における重要なプロセスです.
*これらのプロセスにおけるFUS3遺伝子の役割は調査されています.
* 過去の研究では,FUS3が信号伝達経路に関与することを示唆しています.

研究の目的:

* G1細胞サイクル停止と交配におけるFUS3遺伝子の機能を明らかにする.
* FUS3.3を含む遺伝的相互作用と経路を特定する.

主な方法:

* fus3-1および fus3-2変異体の分析.
*Daf1/whi1 (CLN3) とgpa1ヌル変異を用いた遺伝抑制研究.
*野生型細胞におけるFUS3の過剰発現に関する研究.

主要な成果:

*Fus3変異体はフェロモン曝露時にG1停止の欠陥を示します.
*G1停止欠陥は cln3 の無変異によって抑制されています.
*FUS3は結合を正に調節し,過剰発現はフェロモンの感受性を高め,欠乏は不妊症を引き起こす.
* Fus3のヌル変異はgpa1のヌル変異を抑制し,FUS3を信号伝達経路に配置する.
* FUS3タンパク質は,既知のキナーゼ (CDC28およびKSS1) とホモロジーを共有しています.

結論:

*FUS3は,酵母におけるG1の逮捕と交配に不可欠です.
* FUS3はフェロモン信号伝導経路内で機能する.
*FUS3は結合と細胞サイクル停止のポジティブレギュレータとして作用する.

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