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In vivo and in vitro Studies of Adaptor-clathrin Interaction
Published on: January 26, 2011
int-2オンコプロテインの亜細胞的な運命は,開始コドンの選択によって決定されます
Nature
|February 15, 1990
まとめ
線維細胞成長因子 (FGF) は細胞の成長と分化に影響を与えます. この研究は,新しい,拡張されたInt-2タンパク質形態を特定し,二重の局所化と独特の細胞機能を明らかにしました.
科学分野:
- 分子生物学は分子生物学である.
- 細胞生物学 細胞生物学
- 腫瘍学 腫瘍学
背景:
- 線維細胞成長因子 (FGF) は,細胞の成長と分化において重要な役割を果たします.
- FGFファミリーのメンバーであるマウスint-2遺伝子は,胚の発達と癌に関与しています.
- 以前の研究では,245アミノ酸タンパク質をコードする複数のint-2メッセンジャーRNAクラスを特定しました.
研究 の 目的:
- int-2遺伝子の代替翻訳開始を調査する.
- 新しい,N-末端に拡張されたInt-2タンパク質製品を特徴付けるために.
- 異なるInt-2形態のサブセルラー局所化と機能的影響を決定する.
主な方法:
- COS-1細胞とint-2互補DNAの一時的なトランスフェクション.
- AUGとCUGのコドンから開始されたタンパク質製品の分析.
- 免疫光や細胞分断を用いたサブセルラー局所化研究.
主要な成果:
- フレーム内のCUGコドンで開始された追加のN端末拡張Int-2タンパク質の識別.
- AUGが誘発するInt-2プロダクトは,主に分泌経路に局在しています.
- CUGが誘発したInt-2プロダクトは,核に特異的に局在しています.
結論:
- Int-2オンコタンパク質は,少なくとも2つの異なる形態で生成することができます.
- これらの異なるInt-2形態は,異なる細胞下部位 (分泌経路と核) を表しています.
- 二重の局所化は,Int-2オンコタンパク質が2つの独立したメカニズムを通じてその影響を及ぼし,細胞の行動に異なる影響を及ぼす可能性があることを示唆しています.
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