菌類の小さなRNAは,宿主RNAの干渉経路を乗っ取ることで,植物の免疫を抑制する
Arne Weiberg1, Ming Wang, Feng-Mao Lin
1Department of Plant Pathology and Microbiology, University of California, Riverside, CA 92521, USA.
Science (New York, N.Y.)
|October 5, 2013
まとめ
Botrytis cinereaは小さなRNA (Bc-sRNAs) を使用して,植物免疫遺伝子を抑制し,灰色のカビの病気を可能にします. このクロスキングダムRNA干渉は,高度な真菌の毒性の戦略を強調しています.
科学分野:
- 植物病理学 植物病理学
- 分子生物学は分子生物学である.
- ミコロジー 菌類学
背景:
- Botrytis cinereaは,200種類以上の植物に灰色のカビの病気を引き起こす.
- 菌類の病原体は,宿主の防御を克服するために,しばしば洗練されたメカニズムを採用します.
研究 の 目的:
- 植物免疫におけるBotrytis cinerea小RNA (Bc-sRNA) の役割を調査する.
- Bc-sRNAが宿主RNA干渉 (RNAi) 経路と相互作用するメカニズムを解明する.
主な方法:
- 植物免疫遺伝子を標的とするBc-sRNAの分析.
- アラビドプシスアゴ1変異体を使って,感受性を評価する.
- Botrytis cinerea dcl1 dcl2のダブルミュータントを用いて病原性を評価した.
主要な成果:
- 特定のBc-sRNAは,アラビドプシスとトマトの免疫遺伝子を沈黙させることが判明しました.
- Bc-sRNAは,アルゴナウト1 (AGO1) と相互作用することで,宿主RNAの干渉機構をハイジャックする.
- アラビドプシスのago1変異体はB. cinereaに対する感受性が低下し,dcl1 dcl2変異体は病原性の低下を示した.
結論:
- Botrytis cinereaは,宿主の免疫を抑制するために,毒性の小型のRNAエフェクターを使用します.
- 自然に発生するクロスキングダムRNA干渉は,B. cinerea.の重要なウイルス性メカニズムです.
- この研究は,真菌の病原性および宿主免疫抑制のための高度な戦略を明らかにしています.
関連する概念動画
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This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
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