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関連する概念動画

Hedgehog Signaling Pathway02:33

Hedgehog Signaling Pathway

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The Hedgehog gene (Hh) was first discovered due to its control of the growth of disorganized, hair-like bristles phenotype in Drosophila, much like hedgehog spines. Hh plays a crucial role in the development of organs and the maintenance of homeostasis in both invertebrates and vertebrates. However, while Drosophila has only one Hh protein, mammals have multiple functional Hedgehog proteins - Sonic (Shh), Desert (Dhh), and Indian Hedgehog (Ihh). All of these homologous proteins have adapted to...
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Hedgehog Signaling Pathway02:33

Hedgehog Signaling Pathway

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Interactions Between Signaling Pathways01:19

Interactions Between Signaling Pathways

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Signaling cascades usually lack linearity. Multiple pathways interact and regulate one another, allowing cells to integrate and respond to diverse environmental stimuli.
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
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Assembly of Signaling Complexes01:30

Assembly of Signaling Complexes

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Multiprotein signaling complexes are formed in a dynamic process involving protein-protein interactions at the cytoplasmic domain of transmembrane receptors or enzymatic and non-enzymatic proteins associated with the receptor. These complexes ensure the activation and propagation of intracellular signals that regulate cell functions.
Interaction domains in cell signaling
Interaction domains recognize exposed features of their binding partners containing post-translationally modified sequences,...
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IP3/DAG Signaling Pathway01:11

IP3/DAG Signaling Pathway

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Membrane lipids such as phosphatidylinositol (PI) are precursors for several membrane-bound and soluble second messengers. Specific kinases phosphorylate PI and produce phosphorylated inositol phospholipids. One such inositol phospholipids are the  phosphatidylinositol-4,5 bisphosphate [PI(4,5)P2], present in the inner half of the lipid bilayer. Upon ligand binding, GPCR stimulates Gq proteins to turn on phospholipase Cꞵ. Activated phospholipase Cꞵ cleaves PI(4,5)P2 and...
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PI3K/mTOR/AKT Signaling Pathway01:22

PI3K/mTOR/AKT Signaling Pathway

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The mammalian target of rapamycin  (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1  (mTORC1) and mTOR complex 2  (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast,  mTORC2 consists of a...
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Updated: May 7, 2026

Monitoring Hippo Signaling Pathway Activity Using a Luciferase-based Large Tumor Suppressor LATS Biosensor
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Monitoring Hippo Signaling Pathway Activity Using a Luciferase-based Large Tumor Suppressor LATS Biosensor

Published on: September 13, 2018

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ヒッポのシグナル伝達経路はインタラクトームです.

Young Kwon1, Arunachalam Vinayagam, Xiaoyun Sun

  • 1Department of Genetics, Harvard Medical School, Boston, MA 02115, USA.

Science (New York, N.Y.)
|October 12, 2013
PubMed
まとめ

研究者は,ヒッポの経路のタンパク質相互作用をマッピングし,153のタンパク質と204の相互作用を特定しました. 新しいタンパク質であるLeashは,ヨーク犬を劣化させ,臓器の成長と癌に影響を与えることが判明しました.

科学分野:

  • 細胞生物学 細胞生物学
  • 発達生物学 発達生物学とは
  • 分子生物学は分子生物学である.

背景:

  • Hippo経路は,細胞増殖とアポトーシスを制御することによって,臓器のサイズを調節するために重要です.
  • その重要性にもかかわらず,ヒッポの経路の完全な組成と構造は,まだ完全に理解されていません.

研究 の 目的:

  • ドロソフィラ・ヒッポの経路のための包括的なタンパク質-タンパク質相互作用ネットワーク (Hippo-PPIN) を構築する.
  • ヒッポの経路内の新しいコンポーネントと規制メカニズムを特定する.

主な方法:

  • 既存のHippo経路コンポーネントを餌として利用した質量スペクトロメトリを使用して,Hippo-PPINを生成しました.
  • トランスクリプションのコアクティベーターYorki (Yki) に対する特定されたタンパク質の機能的影響を評価するためにRNA干渉 (RNAi) を採用した.
  • 新しいアルファ-アレスティン・ファミリーメンバー,リーシュを選んで特徴付けました.

主要な成果:

  • 153のタンパク質と204の相互作用を含む高信頼性のHippo-PPINを生成しました.
  • RNAiスクリーニングは,特定されたタンパク質の67%の枯渇が,Yorki (Yki) の活動に正または負の影響を及ぼすことを明らかにしました.

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Identification of Transcription Factor Regulators using Medium-Throughput Screening of Arrayed Libraries and a Dual-Luciferase-Based Reporter
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Culturing and Manipulation of O9-1 Neural Crest Cells
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Culturing and Manipulation of O9-1 Neural Crest Cells

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Last Updated: May 7, 2026

Monitoring Hippo Signaling Pathway Activity Using a Luciferase-based Large Tumor Suppressor LATS Biosensor
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Identification of Transcription Factor Regulators using Medium-Throughput Screening of Arrayed Libraries and a Dual-Luciferase-Based Reporter
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Identification of Transcription Factor Regulators using Medium-Throughput Screening of Arrayed Libraries and a Dual-Luciferase-Based Reporter

Published on: March 27, 2020

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Culturing and Manipulation of O9-1 Neural Crest Cells
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Culturing and Manipulation of O9-1 Neural Crest Cells

Published on: October 9, 2018

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  • リッシュは,リゾソーム経路経由でヨーク犬 (Yki) の分解を促進する新しいレギュレータとして特定されました.

    • 結論:

    • 生成されたHippo-PPINは,Hippo経路の構成と機能を理解するための貴重なリソースを提供します.
    • Leashは,ヨーク犬 (Yki) を対象に退化させることで,ヒッポの経路を否定的に調節する新発見のコンポーネントを表しています.
    • この研究は,正常な臓器の成長におけるヒッポの経路の役割と,がんの発症におけるその影響の理解を深める.