必須遺伝子の遺伝的再コーディングの限界を検証する
M J Lajoie1, S Kosuri, J A Mosberg
1Department of Genetics, Harvard Medical School, Boston, MA 02115, USA.
Science (New York, N.Y.)
|October 19, 2013
まとめ
Escherichia coli の必須遺伝子から希少なコドンを取り除き,遺伝子コードを根本的に変更することは可能である. しかし,ゲノム設計上の制約は,急速なプロトタイプ作成と遺伝子コードの改変のテストの必要性を強調しています.
科学分野:
- 合成生物学 合成生物学とは
- 遺伝子工学 遺伝子工学とは
- 微生物バイオテクノロジー
背景:
- 遺伝子コードを根本的に変更することで,新しい化学的能力と生物学的封じ込めを持つ工学的に作られた生物を可能にします.
- 以前の研究では,UAGのストップコドンの再割り当てが行われていましたが,一般的な感覚コドンの改変は,遺伝子発現における規制的な役割のため,より大きな課題を提示しています.
研究 の 目的:
- 重要な遺伝子を改変することによって遺伝コードを根本的に変更する可能性を評価する.
- ゲノム全体のコドン再配分に関連する課題と制約を調査する.
主な方法:
- エシェリキア・コライ菌で高度に発現する 42 つの必須遺伝子を改変するために選択しました.
- これらの選択された遺伝子から13個の希少コドンのすべてのインスタンスを取り除きました.
- 80種類のエンジニアリングされたE. coli菌株に,残ったすべてのコドンをシャッフルしようとしました.
主要な成果:
- 重要な遺伝子から13個の特定のコドンを全ゲノムにわたって除去する可能性を実証した.
- 遺伝子コードの変異過程に影響を与えるいくつかのゲノム設計の制約を特定した.
結論:
- ゲノム全体のコドン除去は達成可能ですが,設計上の制限を慎重に考慮する必要があります.
- 複雑な遺伝子改変のエンジニアリングのための急速なプロトタイピングと反復テスト戦略の必要性を強調しています.
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