関連する実験動画
Updated: May 6, 2026

06:06
In Vitro Analysis of E3 Ubiquitin Ligase Function
Published on: May 14, 2021
5.3K
HOIPによる線形ユビキチン鎖のリガゼ特異的結合の構造的基礎
Benjamin Stieglitz1, Rohini R Rana1, Marios G Koliopoulos1
1Division of Molecular Structure, MRC-National Institute for Medical Research, The Ridgeway, London NW7 1AA, UK.
Nature
|October 22, 2013
まとめ
線形ユビキチン鎖は,核因子 (NF) -κB.を活性化することによって,免疫と炎症を調節する. HOIP酵素は,HOIPという酵素で
科学分野:
- バイオケミストリーと分子生物学
- セルラー・シグナリング
- ユビキチネーション (Ubiquitination) とは ユビキチネーション (Ubiquitination) とは ユビキチネーション (Ubiquitination) とは
背景:
- 線形ユビキチン鎖は,先天性免疫,炎症,アポトーシスの重要な調節体である.
- 線形ユビキチン鎖組成複合体 (LUBAC) の触媒成分であるHOIPは,これらの鎖を合成する.
- HOIPのようなRBR E3リガゼは,ユビキチネーションにおけるRING/HECTハイブリッドとして機能する.
研究 の 目的:
- HOIPの触媒活性と線形ユビキチン鎖の形成の構造的基礎を解明する.
- HOIPがチェーンアセンブリのためのユビキチンを具体的に認識し,活性化する方法を理解する.
- 線形ユビキチネーションにおけるLUBACの特異性の構造的決定因子を決定する.
主な方法:
- X線結晶学を用いてHOIPの触媒核の構造を決定した.
- 構造は,アポ形式と,ユビキチンとの複合体の両方で得られた.
- ユビキチン結合プラットフォームとユビキチン転送機構の分析.
主要な成果:
- HOIPの触媒コアの結晶構造は,ユビキチン結合プラットフォームを形成する新しい折り目を明らかにしました.
- 亜鉛指を備えたこのプラットフォームは,核愛性の攻撃のためにユビキチンを正しく指向します.
- ユビキチン伝達複合体のユニークなスナップショットは,ドナーと受容体のユビキチンの触媒性システインとの近接を示した.
結論:
- 決定された構造は,HOIPによる線形ユビキチン鎖合成のメカニズムを説明します.
- HOIPのユニークなユビキチン結合プラットフォームは,ユビキチンを指向し,特異性を確保するために不可欠です.
- これらの構造的な洞察は,NF-κB経路の活性化とLUBACの機能を理解するために不可欠です.
関連する概念動画
Mechanism of Conjugation
1.6K
Bacterial conjugation is a mechanism of horizontal gene transfer that enables the exchange of genetic material between bacterial cells through direct contact. This process is facilitated by a donor cell carrying a conjugative plasmid, which encodes genes necessary for pilus formation, DNA replication, and transfer. The conjugative plasmid plays a central role in initiating and executing the transfer of genetic material.The tra region of the conjugative plasmid encodes proteins responsible for...
1.6K
Ligand Binding and Linkage
4.4K
Allosteric proteins have more than one ligand binding site; the binding of a ligand to any of these sites influences the binding of ligands to the other sites. When a protein is allosteric, its binding sites are called coupled or linked. In the case of enzymes, the site that binds to the substrate is known as the active site and the other site is known as the regulatory site. When a ligand binds to the regulatory site, this leads to conformational changes in the protein that can influence...
4.4K
Ligand Binding and Linkage
3.0K
3.0K
Covalently Linked Protein Regulators
8.2K
Proteins can undergo many types of post-translational modifications, often in response to changes in their environment. These modifications play an important role in the function and stability of these proteins. Covalently linked molecules include functional groups, such as methyl, acetyl, and phosphate groups, and also small proteins, such as ubiquitin. There are around 200 different types of covalent regulators that have been identified.
These groups modify specific amino acids in a protein....
These groups modify specific amino acids in a protein....
8.2K
Protein Complexes with Interchangeable Parts
2.1K
Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order...
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order...
2.1K
Conservative Site-specific Recombination and Phase Variation
5.7K
Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
The recognition sites for Cre recombinase called LoxP...
5.7K

