二つのエステル結合のフォスフォラスへの連続水解を触媒化するサイクリック・フォスフォディエステラーゼの発見

関連する実験動画

Updated: May 6, 2026

A Purification and In Vitro Activity Assay for a pppGpp Synthetase from Clostridium difficile
09:53

A Purification and In Vitro Activity Assay for a pppGpp Synthetase from Clostridium difficile

Published on: November 3, 2018

8.0K

Swapnil V Ghodge1, Jennifer A Cummings, Howard J Williams

  • 1Department of Chemistry, Texas A&M University , P.O. Box 30012, College Station, Texas 77843-3012, United States.

Journal of the American Chemical Society
|October 24, 2013
PubMed
まとめ

研究者らは,PRcPのようなサイクルフォスフォディエステルを分解する新しい酵素であるサイクルフォスファートジヒドローラゼ (cPDH) を発見した. この酵素は,無機リン酸とリボス-5-リン酸を生成し,オルガノフォスフォン酸代謝を理解するために不可欠です.

さらに関連する動画

Chemical Triphosphorylation of Oligonucleotides13:19

Chemical Triphosphorylation of Oligonucleotides

Published on: June 2, 2022

5.1K
Nucleoside Triphosphates - From Synthesis to Biochemical Characterization15:22

Nucleoside Triphosphates - From Synthesis to Biochemical Characterization

Published on: April 3, 2014

16.7K

関連する概念動画

Phosphodiester Linkages01:01

Phosphodiester Linkages

Overview
Phosphodiester bond forms when a phosphoric acid molecule (H3PO4) links with two hydroxyl groups (–OH) of two other molecules, forming two ester bonds. Two water molecules are released in this process. The phosphodiester bond is commonly found in nucleic acids (DNA and RNA) and plays a critical role in their structure and function.
Phosphodiester Bonds Link Nucleotides Together
DNA and RNA are polynucleotides or long chains of nucleotides that are linked together. A nucleotide is...
99.2K
Phosphorylation01:02

Phosphorylation

The addition or removal of phosphate groups from proteins is the most common chemical modification that regulates cellular processes. These modifications can affect the structure, activity, stability, and localization of proteins within cells as well as their interactions with other proteins.
During phosphorylation, protein kinases transfer the terminal phosphate group of ATP to specific amino acid side chains of substrate proteins. Serine, threonine, and tyrosine are the most commonly...
44.8K
Protein Kinases and Phosphatases02:54

Protein Kinases and Phosphatases

Proteins undergo chemical modifications that trigger changes in the charge, structure, and conformation of the proteins. Phosphorylation, acetylation, glycosylation, nitrosylation, ubiquitination, lipidation, methylation, and proteolysis are various protein modifications that regulate protein activity. Such modifications are usually enzyme-driven.
Protein kinases
Many proteins in the cell are regulated by phosphorylation, the addition of a phosphate group. A family of enzymes called kinases...
12.2K
The Phosphorus Cycle01:21

The Phosphorus Cycle

Unlike carbon, water, and nitrogen, phosphorus is not present in the atmosphere as a gas. Instead, most phosphorus in the ecosystem exists as compounds, such as phosphate ions (PO43-), found in soil, water, sediment and rocks. Phosphorus is often a limiting nutrient (i.e., in short supply). Consequently, phosphorus is added to most agricultural fertilizers, which can cause environmental problems related to runoff in aquatic ecosystems.
35.1K
Hydrolysis of ATP01:08

Hydrolysis of ATP

The bonds of adenosine triphosphate (ATP) can be broken through the addition of water, releasing one or two phosphate groups in an exergonic process called hydrolysis. This reaction liberates the energy in the bonds for use in the cell—for instance, to synthesize proteins from amino acids.
If one phosphate group is removed, a molecule of ADP—adenosine diphosphate—remains, along with inorganic phosphate. ADP can be further hydrolyzed to AMP—adenosine...
72.4K
Hydrolysis of ATP01:08

Hydrolysis of ATP

7.1K