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関連する概念動画

RNA Interference01:23

RNA Interference

24.4K
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
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Experimental RNAi02:15

Experimental RNAi

6.5K
RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
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siRNA - Small Interfering RNAs02:30

siRNA - Small Interfering RNAs

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Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the...
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Small interfering RNAs (siRNA)02:30

Small interfering RNAs (siRNA)

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Translational Regulation01:29

Translational Regulation

893
Translational regulation in prokaryotes ensures efficient protein synthesis by controlling ribosome access to mRNA. This regulation is mediated by secondary RNA structures, including translational riboswitches, RNA thermometers, and small RNAs (sRNAs), which respond to intracellular and environmental signals to modulate gene expression.Translational RiboswitchesRiboswitches in the leader region of mRNAs can regulate translation by altering the accessibility of the Shine-Dalgarno (SD) sequence,...
893
Nonsense-mediated mRNA Decay02:27

Nonsense-mediated mRNA Decay

9.4K
The Upf proteins that carry out nonsense-mediated decay (NMD) are found in all eukaryotic organisms, including humans. Each protein has an individual role, but they need to work in collaboration. Upf1 is an ATP-dependent RNA helicase that unwinds the RNA helix. Because Upf1 can unwind any RNA, Upf2 and Upf3 are required to help Upf1 discriminate between nonsense and normal mRNAs.
Usually, Upf3 binds to an Exon Junction Complex (EJC) at mRNA splice sites. If a ribosome fully translates the mRNA,...
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MISSION esiRNA for RNAi Screening in Mammalian Cells
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MISSION esiRNA for RNAi Screening in Mammalian Cells

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コンピュータースクリーニングによる短い干渉RNAガイドストランド変形剤.

Kazumitsu Onizuka1, Jason G Harrison, Alexi A Ball-Jones

  • 1Department of Chemistry, University of California, Davis , One Shields Ave, Davis, California 95616, United States.

Journal of the American Chemical Society
|October 25, 2013
PubMed
まとめ
この要約は機械生成です。

短い干渉RNA (siRNA) に対する化学的改変により,それらの薬剤の可能性が強化されます. 研究者らは,siRNAガイドストランドの新しい機能的修正を発見し,遺伝子サイレンシングの効率を向上させました.

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A Reporter Based Cellular Assay for Monitoring Splicing Efficiency
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Using In Vitro and In-cell SHAPE to Investigate Small Molecule Induced Pre-mRNA Structural Changes
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Using In Vitro and In-cell SHAPE to Investigate Small Molecule Induced Pre-mRNA Structural Changes

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関連する実験動画

Last Updated: May 6, 2026

MISSION esiRNA for RNAi Screening in Mammalian Cells
15:31

MISSION esiRNA for RNAi Screening in Mammalian Cells

Published on: May 12, 2010

18.8K
A Reporter Based Cellular Assay for Monitoring Splicing Efficiency
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A Reporter Based Cellular Assay for Monitoring Splicing Efficiency

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Using In Vitro and In-cell SHAPE to Investigate Small Molecule Induced Pre-mRNA Structural Changes
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科学分野:

  • バイオケミストリー バイオケミストリー
  • 分子生物学は分子生物学である.
  • ドラッグ・ディスカバリー・ドリッグ・ディスカバリー・ドリッグ・ディスカバリー・ドリッグ・ディスカバリー

背景:

  • 短い干渉RNA (siRNAs) は,さまざまな病気を標的とした強力な治療薬です.
  • ネイティブRNAの構造は,siRNA薬の開発に限界を提示し,化学的修正を必要とします.
  • 人間のアルゴナウト2 (hAgo2) タンパク質は,RNA干渉 (RNAi) 経路の有効性にとって中心的なものです.

研究 の 目的:

  • siRNAガイドストランドの5eeal-endの機能的化学的修正を発見するために.
  • ハゴ2のコンピューティングスクリーニングと構造データを改変発見の指針として利用する.
  • 強化されたsiRNA療法のためのヌクレオチドアナログの範囲を拡大する.

主な方法:

  • 潜在的な siRNA 改変の構造誘導計算スクリーニング.
  • 人間のAgo2 (hAgo2) の高解像度構造を利用しています.
  • RNAの1,2,3-トリアゾル-4-イル塩基とピューリン誘導体を合成し,試験する.

主要な成果:

  • siRNAガイドストランドの5eal-endヌクレオチドには,必ずしもワトソン・クリックのH結合ではなく, hAgo2結合部位における適切な形状の互換性が必要です.
  • CuAAC反応によって合成される1,2,3-トリアゾル-4-イル基は,siRNA 5eal-end.で有効な改変である.
  • 改変されたフーグスティーン面またはN2置換剤を有するピューリン誘導体は,5eal-end改変には適さないことが判明しました.
  • ワトソン・クリックのH結合能力が欠けている1,2,3-トリアゾル-4-イル基は,ガイドストランドの位置12で有効性を示した.

結論:

  • 機能的なsiRNAの改変は,構造主導の計算方法によって発見することができます.
  • 1,2,3-トリアゾル-4-イル塩基などの新型ヌクレオチドアナログは,siRNA薬剤設計の新たな可能性を提供します.
  • hAgo2-siRNAの相互作用を理解することは,より効果的なsiRNA治療法の開発の鍵です.