関連する実験動画

Updated: May 5, 2026

Direct Restart of a Replication Fork Stalled by a Head-On RNA Polymerase
07:27

Direct Restart of a Replication Fork Stalled by a Head-On RNA Polymerase

Published on: April 30, 2010

16.0K

E. coliおよびHeLa細胞からの不活性サブユニットを用いてRNAase P活性を再構成

H A Gold, S Altman

    Cell
    |January 31, 1986
    PubMed
    まとめ

    研究者は,非活性RNAおよびタンパク質成分を使用して,活性HeLa細胞RNAaseP酵素複合体を再構成した. この方法は,プロカリオットRNAase P.から異なるHeLa細胞から機能性RNAとタンパク質を浄化するのに役立ちます.

    さらに関連する動画

    In vitro Reconstitution of the Active T. castaneum Telomerase09:25

    In vitro Reconstitution of the Active T. castaneum Telomerase

    Published on: July 14, 2011

    11.1K
    Semi-quantitative Detection of RNA-dependent RNA Polymerase Activity of Human Telomerase Reverse Transcriptase Protein08:26

    Semi-quantitative Detection of RNA-dependent RNA Polymerase Activity of Human Telomerase Reverse Transcriptase Protein

    Published on: June 12, 2018

    11.7K

    関連する概念動画

    Bacterial RNA Polymerase00:43

    Bacterial RNA Polymerase

    Unlike eukaryotes, bacteria use a single RNA Polymerase (RNAP) to transcribe all genes. The different subunits of bacterial RNAPhave distinct functions. The multisubunit structure of the bacterial RNAP helps the enzyme to maintain catalytic function, facilitate assembly, interact with DNA and RNA, and self-regulate its activity.
    In most genes, the transcription site is a single base present upstream of the coding sequence. Though RNAP is a catalytically efficient enzyme, it does not recognize...
    20.0K
    Eukaryotic RNA Polymerases00:58

    Eukaryotic RNA Polymerases

    RNA Polymerase (RNAP) is conserved in all animals, with bacterial, archaeal, and eukaryotic RNAPs sharing significant sequence, structural, and functional similarities. Among the three eukaryotic RNAPs, RNA Polymerase II is most similar to bacterial RNAP in terms of both structural organization and folding topologies of the enzyme subunits. However, these similarities are not reflected in their mechanism of action.
    All three eukaryotic RNAPs require specific transcription factors, of which the...
    17.3K
    Ribozymes02:47

    Ribozymes

    The term ribozyme is used for RNA that can act as an enzyme. Ribozymes are mainly found in selected viruses, bacteria, plant organelles, and lower eukaryotes. Ribozymes were first discovered in 1982 when Tom Cech’s laboratory observed Group I introns acting as enzymes. This was shortly followed by the discovery of another ribozyme, Ribonulcease P, by Sid Altman’s laboratory. Both Cech and Altman received the Nobel Prize in chemistry in 1989 for their work on ribozymes.
    Ribozymes can...
    10.0K
    Transcription Initiation01:47

    Transcription Initiation

    Initiation is the first step of transcription in eukaryotes. Prokaryotic RNA Polymerase (RNAP) can bind to the template DNA and start transcribing. On the other hand, transcription in eukaryotes requires additional proteins, called transcription factors, to first bind to the promoter region in the DNA template. This binding helps recruit the specific RNAP that can assemble on the DNA and start transcription.
    The promoters and enhancers and their accessory proteins allow tight regulation of...
    17.0K