Jove
Visualize
お問い合わせ
JoVE
x logofacebook logolinkedin logoyoutube logo
JoVEについて
概要リーダーシップブログJoVEヘルプセンター
著者向け
出版プロセス編集委員会範囲と方針査読よくある質問投稿
図書館員向け
推薦の声購読アクセスリソース図書館諮問委員会よくある質問
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experimentsアーカイブ
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教員リソースセンター教員サイト
利用規約
プライバシーポリシー
ポリシー

関連する概念動画

Complexometric Titration: Ligands00:43

Complexometric Titration: Ligands

2.6K
Different monodentate and polydentate ligands are used as complexing agents in complexometric titration reactions. The formation of complexes by mono- and bidentate ligands involves two or more intermediate steps, limiting their use as complexing agents. In comparison, polydentate ligands can form complexes with metal ions in a single-step process, facilitating sharper end points. This means polydentate ligands, such as amino carboxylic acid derivatives, are most commonly employed in...
2.6K
Metal-Ligand Bonds02:51

Metal-Ligand Bonds

19.3K
The hemoglobin in the blood, the chlorophyll in green plants, vitamin B-12, and the catalyst used in the manufacture of polyethylene all contain coordination compounds. Ions of the metals, especially the transition metals, are likely to form complexes.
In these complexes, transition metals form coordinate covalent bonds, a kind of Lewis acid-base interaction in which both of the electrons in the bond are contributed by a donor (Lewis base) to an electron acceptor (Lewis acid). The Lewis acid in...
19.3K
Complexation Equilibria: The Chelate Effect01:19

Complexation Equilibria: The Chelate Effect

1.7K
In complexation reactions, metal atoms or cations interact with ligands to form donor-acceptor adducts called metal complexes. Ligands that bind through one donor site are monodentate, ligands with two donor sites are bidentate, and those with more than two donor sites are polydentate ligands. For example, ethylene diamine is a bidentate ligand that binds through two nitrogen donor atoms, forming a five-membered ring. EDTA is a polydentate ligand that binds through four oxygen and two nitrogen...
1.7K
Ligand Binding Sites02:40

Ligand Binding Sites

11.9K
Proteins are dynamic macromolecules that carry out a wide variety of essential processes; however, the activities of most proteins depend on their interactions with other molecules or ions, known as ligands.
Protein-ligand interactions are quite specific; even though numerous potential ligands surround a cellular protein at any given time, only a particular ligand can bind to that protein. Moreover, a ligand binds only to a dedicated area on the surface of the protein, known as the...
11.9K
EDTA: Chemistry and Properties01:22

EDTA: Chemistry and Properties

4.2K
Polydentate ligands are most widely used in complexometric titrations because they form more stable complexes with the metal ions than mono- or bidentate ligands due to the chelate effect. Examples of polydentate ligands are ethylenediaminetetraacetic acid (EDTA), crown ethers, and cryptands. The most important feature of optimal polydentate ligands is the ability to form 1:1 complexes in a single-step process. Amino carboxylic acid derivatives are frequently used as complexing agents. EDTA is...
4.2K
Ligand Binding and Linkage00:49

Ligand Binding and Linkage

4.4K
Allosteric proteins have more than one ligand binding site; the binding of a ligand to any of these sites influences the binding of ligands to the other sites. When a protein is allosteric, its binding sites are called coupled or linked.  In the case of enzymes, the site that binds to the substrate is known as the active site and the other site is known as the regulatory site. When a ligand binds to the regulatory site, this leads to conformational changes in the protein that can influence...
4.4K

こちらも読む

関連記事

共著者、ジャーナル、引用グラフによってこの研究に関連する記事。

並び替え
Same author

Design Rules for Selective Peptide Amphiphile-Gold Nanoparticle Interactions from Atomistic Simulations.

Langmuir : the ACS journal of surfaces and colloids·2026
Same author

tRNA-deacylase-directed discovery of biosynthetic pathways.

Nature chemistry·2026
Same author

Exploring the Modularity of Triphenylphosphine-Containing Polymers as Diverse Transition Metal Catalysts.

ACS macro letters·2026
Same author

Biosynthesis of Minimal C-Phycocyanin Chromophore Assemblies in <i>E. coli</i> Provides a Platform to Dissect Protein-Mediated Tuning of Exciton Transfer.

Journal of the American Chemical Society·2026
Same author

Engineered MS2 Virus Capsids for Cellular Display of Peptide Antigens.

ACS chemical biology·2025
Same author

Site-selective protein editing by backbone extension acyl rearrangements.

Nature chemical biology·2025

関連する実験動画

Updated: May 6, 2026

Creating Highly Specific Chemically Induced Protein Dimerization Systems by Stepwise Phage Selection of a Combinatorial Single-Domain Antibody Library
10:17

Creating Highly Specific Chemically Induced Protein Dimerization Systems by Stepwise Phage Selection of a Combinatorial Single-Domain Antibody Library

Published on: January 14, 2020

7.3K

選択的クロム (VI) リガンドは,組合せペプトイドライブラリを使用して識別されました.

Abigail S Knight1, Effie Y Zhou, Jeffrey G Pelton

  • 1Department of Chemistry, University of California , 724 Latimer Hall, Berkeley, California 94720, United States.

Journal of the American Chemical Society
|November 8, 2013
PubMed
まとめ
この要約は機械生成です。

研究者は,水から有毒な六価クロム[Cr(VI]を選択的に捕獲するために,ペプトイドベースの新しいリガンドを開発しました. これらのリガンドは,環境水サンプルにおけるCr (VI) の汚染を修復するための費用対効果の高い解決策を提供します.

さらに関連する動画

Covalent Fragment Screening Using the Quantitative Irreversible Tethering Assay
06:17

Covalent Fragment Screening Using the Quantitative Irreversible Tethering Assay

Published on: February 28, 2025

1.3K
Achieving Efficient Fragment Screening at XChem Facility at Diamond Light Source
08:35

Achieving Efficient Fragment Screening at XChem Facility at Diamond Light Source

Published on: May 29, 2021

7.5K

関連する実験動画

Last Updated: May 6, 2026

Creating Highly Specific Chemically Induced Protein Dimerization Systems by Stepwise Phage Selection of a Combinatorial Single-Domain Antibody Library
10:17

Creating Highly Specific Chemically Induced Protein Dimerization Systems by Stepwise Phage Selection of a Combinatorial Single-Domain Antibody Library

Published on: January 14, 2020

7.3K
Covalent Fragment Screening Using the Quantitative Irreversible Tethering Assay
06:17

Covalent Fragment Screening Using the Quantitative Irreversible Tethering Assay

Published on: February 28, 2025

1.3K
Achieving Efficient Fragment Screening at XChem Facility at Diamond Light Source
08:35

Achieving Efficient Fragment Screening at XChem Facility at Diamond Light Source

Published on: May 29, 2021

7.5K

科学分野:

  • 環境化学 環境化学
  • マテリアルサイエンス 材料科学
  • バイオテクノロジー バイオテクノロジー

背景:

  • 六価クロム (Cr(VI) は,世界的に浸透している水汚染物質です.
  • 現在のCr (VI) の修復戦略には,費用対効果と効率性が欠けている.
  • 主な課題は,水溶液中の多くの他のイオンとCr (VI) を結合できる選択性リガンドの欠如です.

研究 の 目的:

  • 選択的なCr (VI) 結合のためのリガンド候補のペプトイドベースのライブラリを設計し,適用する.
  • 中性pHのCr (VI) に対して高い親和性と特異性を有するペプトイド配列を特定し,特徴づけること.
  • これらのペプトイドリガンドが,Cr (VI) で汚染された環境水サンプルを修復する効果を評価する.

主な方法:

  • ペプトイド配列のライブラリが合成され,Cr (VI) 結合のためにスクリーニングされました.
  • 特定された配列の親和度定位には,UV-VIS光譜を用いた.
  • Cr (VI) -リガンド複合体の詳細な特徴を特定するために,NMRスペクトロスコピーを用いた.
  • ペプトイド配列は,修復実験のために固相樹脂に固定されました.

主要な成果:

  • 挑戦的な条件 (中性pH,過剰イオン) の下でCr (VI) を結合できる11のペプトイド配列が特定されました.
  • アフィニティ比較は,選択された配列の間で重要な結合能力を明らかにした.
  • 特徴化は,調整相互作用と特異性決定因子を明らかにした.
  • 合成したペプトイド樹脂は,シミュレートされた汚染水サンプルにおけるCr (VI) レベルをEPAに適合する範囲に効果的に低下させた.

結論:

  • ペプトイドベースのリガンドは,Cr (VI) のような有毒な金属イオンに対する有望な新種のセレクティブケレーターである.
  • これらのリンガンドは,クロム汚染の環境修復のための費用対効果的かつ効率的なアプローチを提供します.
  • この研究は,ペプトイド配列が先進的な環境修復材料の開発のための多用途な構成要素としての可能性を強調しています.