Jove
Visualize
お問い合わせ
JoVE
x logofacebook logolinkedin logoyoutube logo
JoVEについて
概要リーダーシップブログJoVEヘルプセンター
著者向け
出版プロセス編集委員会範囲と方針査読よくある質問投稿
図書館員向け
推薦の声購読アクセスリソース図書館諮問委員会よくある質問
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experimentsアーカイブ
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教員リソースセンター教員サイト
利用規約
プライバシーポリシー
ポリシー

関連する概念動画

Experimental RNAi02:15

Experimental RNAi

6.5K
RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
6.5K
siRNA - Small Interfering RNAs02:30

siRNA - Small Interfering RNAs

13.4K
Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the...
13.4K
RNA Interference01:23

RNA Interference

24.4K
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
24.4K
piRNA - Piwi-interacting RNAs02:57

piRNA - Piwi-interacting RNAs

6.1K
PIWI-interacting RNAs, or piRNAs, are the most abundant short non-coding RNAs. More than 20,000 genes have been found in humans that code for piRNAs while only 2000 genes have been found for miRNAs. piRNAs can act at the transcriptional and post-transcriptional levels and have a vital role in silencing transposable elements present in germ cells. They are also involved in epigenetic silencing and activation. Previously, they were thought to function only in germ cells but new evidence suggests...
6.1K
Translational Regulation01:29

Translational Regulation

893
Translational regulation in prokaryotes ensures efficient protein synthesis by controlling ribosome access to mRNA. This regulation is mediated by secondary RNA structures, including translational riboswitches, RNA thermometers, and small RNAs (sRNAs), which respond to intracellular and environmental signals to modulate gene expression.Translational RiboswitchesRiboswitches in the leader region of mRNAs can regulate translation by altering the accessibility of the Shine-Dalgarno (SD) sequence,...
893
Cis-regulatory Sequences02:02

Cis-regulatory Sequences

9.5K
Cis-regulatory sequences are short fragments of non-coding DNA that are present on the same chromosomes as the genes that they regulate. These fragments serve as binding sites for transcriptional regulators, proteins that are responsible for controlling gene transcription and differential gene expression across cell types in eukaryotes. Cis-regulatory sequences can be close to the gene of interest or thousands of bases away in the DNA sequence; however, those sequences that are further away are...
9.5K

こちらも読む

関連記事

共著者、ジャーナル、引用グラフによってこの研究に関連する記事。

並び替え
Same author

NUPACK: Computational Nucleic Acid Analysis and Design.

ACS synthetic biology·2026
Same author

Models and Algorithms for Equilibrium Analysis of Mixed-Material Nucleic Acid Systems.

ACS synthetic biology·2026
Same author

<i>k</i> -spaces: Mixtures of Gaussian latent variable models.

bioRxiv : the preprint server for biology·2025
Same author

Models and Algorithms for Equilibrium Analysis of Mixed-Material Nucleic Acid Systems.

bioRxiv : the preprint server for biology·2025
Same author

Expression, purification, and characterization of diacylated Lipo-YcjN from Escherichia coli.

The Journal of biological chemistry·2024
Same author

Expression, purification, and characterization of diacylated Lipo-YcjN from <i>Escherichia coli</i>.

bioRxiv : the preprint server for biology·2024

関連する実験動画

Updated: May 6, 2026

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation
09:26

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation

Published on: December 29, 2021

3.9K

条件付きDicer基板形成は,小さな条件付きRNAとの形状と配列伝導によるものです.

Lisa M Hochrein1, Maayan Schwarzkopf, Mona Shahgholi

  • 1Department of Chemical Engineering, ‡Department of Biology, ∥Department of Chemistry, §Department of Bioengineering, and ⊥Department of Computing and Mathematical Sciences, California Institute of Technology , Pasadena, California 91125, United States.

Journal of the American Chemical Society
|November 14, 2013
PubMed
まとめ

研究者は,条件付きRNA干渉 (RNAi) のための小さな条件付きRNA (scRNA) を設計した. これらのscrRNAは,特定の検出標的が存在する場合にのみ,遺伝子の静止を可能にし,遺伝子のノックダウンに対する空間時間的な制御を提供します.

さらに関連する動画

Gene Digital Circuits Based on CRISPR-Cas Systems and Anti-CRISPR Proteins
10:46

Gene Digital Circuits Based on CRISPR-Cas Systems and Anti-CRISPR Proteins

Published on: October 18, 2022

1.9K
Ubiquitous and Tissue-specific RNA Targeting in Drosophila Melanogaster using CRISPR/CasRx
06:37

Ubiquitous and Tissue-specific RNA Targeting in Drosophila Melanogaster using CRISPR/CasRx

Published on: February 5, 2021

4.3K

関連する実験動画

Last Updated: May 6, 2026

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation
09:26

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation

Published on: December 29, 2021

3.9K
Gene Digital Circuits Based on CRISPR-Cas Systems and Anti-CRISPR Proteins
10:46

Gene Digital Circuits Based on CRISPR-Cas Systems and Anti-CRISPR Proteins

Published on: October 18, 2022

1.9K
Ubiquitous and Tissue-specific RNA Targeting in Drosophila Melanogaster using CRISPR/CasRx
06:37

Ubiquitous and Tissue-specific RNA Targeting in Drosophila Melanogaster using CRISPR/CasRx

Published on: February 5, 2021

4.3K

科学分野:

  • 分子生物学は分子生物学である.
  • RNAセラピュティックス
  • 合成生物学 合成生物学とは

背景:

  • RNA干渉 (RNAi) は,小さな干渉RNA (siRNAs) を遺伝子ノックダウンに用いるが,その構成活動が時空制御を制限する.
  • 遺伝子サイレンスに対する正確な制御を達成することは,治療的応用と生物学的研究において極めて重要です.

研究 の 目的:

  • 条件付きRNAiを可能にする小型条件付きRNA (scRNA) を設計し,特定のmRNA標的の存在に反応する.
  • 遺伝子の静止を空間時間的に制御するシステムを開発し,特定の組織と時間に対してノックダウンを制限する.

主な方法:

  • 条件付きDicer基板形成のための多様なscRNA機構を設計し,実験的に検証しました.
  • エンジニアリングされたscRNAはmRNAの"検出標的"と結合し,信号を変換してDicer基板を形成し,独立したmRNAの"静止標的"を標的にします.
  • 条件付きDicer基板生成とsiRNA生成を評価するために,in vitro研究を利用した.

主要な成果:

  • 強烈なOFF/ON条件応答を示し,標的結合の検出時にDicer基板生産が10倍以上増加しました.
  • シグナル伝導産物のみがDicer.によって効率的に処理されることを保証するために,最適化されたscRNA設計 (寸法化,化学改変) を行いました.
  • 反応物質の安定性,触媒機構,分子自己組み立てなど,scRNA信号伝導のための様々な設計原理を探求した.

結論:

  • 条件付きRNAiが可能なscRNAを成功裏に開発し,遺伝子の静止を空間時間的に制御するメカニズムを提供した.
  • エンジニアリングされたscRNAは,精密な遺伝子ノックダウンのための汎用性のあるプラットフォームを提供し,研究と治療における潜在的な応用があります.
  • scRNAの設計原理のさらなる探求は,遺伝子調節のための洗練された分子論理システムの開発を向上させることができます.