EMREは,ミトコンドリアのカルシウムユニポーター複合体の重要な成分です
Yasemin Sancak1, Andrew L Markhard, Toshimori Kitami
1Department of Molecular Biology, Massachusetts General Hospital, Department of Systems Biology, Harvard Medical School, Boston, MA, USA.
まとめ
エッセンシャルMCUレギュレータ (EMRE) タンパク質は,ミトコンドリアカルシウム吸収に不可欠です. EMREは,MICU1/MICU2によるカルシウム検出と,MCUによるカルシウム伝導を橋渡しし,ユニポーターチャネル活動を可能にします.
科学分野:
- ミトコンドリア生物学 ミトコンドリア生物学
- 分子および細胞生理学
- バイオケミストリー バイオケミストリー
背景:
- ミトコンドリアカルシウムユニポーター (MCU) 複合体は,カルシウムホメオスタシスを調節する.
- キーコンポーネントには,MCU,MICU1,MICU2が含まれていますが,ホロコンプレックス全体の構成は不明でした.
研究 の 目的:
- ミトコンドリアのユニポーターホロコンプレックス (ユニプレックス) の分子組成を完全に特徴付けるために.
- ユニプレックスの中で,これまで特徴づけられなかったタンパク質の機能を明らかにする.
主な方法:
- ユニプレックス複合体のアフィニティ浄化.
- タンパク質の成分を特定するための定量的質量スペクトロメトリ.
- 特定されたタンパク質の存在または欠如におけるユニポーターチャネル活動の評価.
主要な成果:
- MICU1,MICU2,MCU,MCUb,および新しいタンパク質,エッセンシャルMCUレギュレータ (EMRE) を特定しました.
- EMREは,ユニポーター活動に不可欠な小さなトランスメブランタンパク質です.
- EMREは,MCUとカルシウム感知タンパク質MICU1/MICU2.2の相互作用のために必要です.
結論:
- EMREは,ミトコンドリアカルシウムユニポーター複合体の重要な成分です.
- EMREは,カルシウム感知とカルシウム伝導を結びつける分子ブリッジとして機能します.
- EMREは,ミトコンドリアのカルシウム吸収に不可欠です.
さらに関連する動画
関連する概念動画
Protein Transport into the Inner Mitochondrial Membrane
3.5K
Nuclear encoded mitochondrial precursors are imported to the inner membrane in a multistep process involving two separate translocons, TIM22 and TIM23. TIM23 is a cation-selective pore that remains closed by the N terminal segment of the protein. Negative charges on the TIM23 act as a receptor for the incoming precursor, pulling the positively charged matrix-targeting sequence for peptide insertion and translocation.
Transport of mitochondrial precursors across the TIM23 channel is driven by...
Transport of mitochondrial precursors across the TIM23 channel is driven by...
3.5K
The Inner Mitochondrial Membrane
3.9K
The inner mitochondrial membrane is the primary site of ATP synthesis. The inner membrane domain that forms a smooth layer adjacent to the outer membrane is called the inner boundary membrane. This domain contains membrane transporters that drive metabolites in and out of the mitochondria. In contrast, the inner membrane network that invaginates into the matrix space is called the cristae membrane. This domain accounts for principle mitochondrial function as it accommodates the protein...
3.9K
Protein Translocation Machinery on the ER Membrane
5.6K
The translocon complex situated on the ER membrane is the main gateway for the protein secretory pathway. It facilitates the transport of nascent peptides into the ER lumen and their insertion into the ER membrane.
Sec61 protein conducting channel
In eukaryotes, the translocon complex comprises a core heterotrimeric translocator channel called the Sec61 complex. This channel includes three transmembrane proteins, Sec61α, Sec61β, and Sec61γ, and is the largest subunit of the...
Sec61 protein conducting channel
In eukaryotes, the translocon complex comprises a core heterotrimeric translocator channel called the Sec61 complex. This channel includes three transmembrane proteins, Sec61α, Sec61β, and Sec61γ, and is the largest subunit of the...
5.6K
Translocation of Proteins into the Mitochondria
8.8K
Mitochondrial precursors are translocated to the internal subcompartments via independent mechanisms involving distinct protein machineries called translocases.
Sorting of outer membrane proteins:
Mitochondrial outer membrane proteins are of two types: the transmembrane, beta-barrel porins, and the membrane-anchored, alpha-helical proteins. Beta-barrel porin precursors are translocated by the TOM complex and inserted into the outer mitochondrial membrane by the SAM complex. In contrast,...
Sorting of outer membrane proteins:
Mitochondrial outer membrane proteins are of two types: the transmembrane, beta-barrel porins, and the membrane-anchored, alpha-helical proteins. Beta-barrel porin precursors are translocated by the TOM complex and inserted into the outer mitochondrial membrane by the SAM complex. In contrast,...
8.8K
Mitochondrial Membranes
11.7K
A single mitochondrion is a bean-shaped organelle enclosed by a double-membrane system. The outer membrane of mitochondria is smooth and contains many porins - the integral membrane transporters. Porins enable free diffusion of ions and small uncharged molecules through the outer mitochondrial membrane but limit the transport of molecules larger than 5000 Daltons. Further, the outer mitochondrial membrane forms a unique structure called membrane contact sites with other subcellular organelles,...
11.7K
Mitochondrial Membranes
2.1K
2.1K


