関連する実験動画
Updated: Aug 5, 2026
08:39
Scale-Up of Mammalian Cell Culture using a New Multilayered Flask
Published on: December 5, 2011
まとめ
このレビューは,哺乳類の細胞に焦点を当てて,大規模な細胞培養のアプリケーション,課題,および将来の可能性をカバーしています. 確立された商業プロセスと,この分野における新興イノベーションを強調しています.
科学分野:
- バイオテクノロジー バイオテクノロジー
- 細胞生物学 細胞生物学
- バイオプロセッシング
背景:
- 大規模な細胞培養は,生物学的および医薬品の生産に不可欠です.
- 商用プロセスはしばしば独占的であり,詳細な公開データを制限しています.
- 哺乳類の細胞培養は,産業用アプリケーションを支配しています.
研究 の 目的:
- 大規模細胞培養の現状を見直す.
- その多様な応用,固有の問題,そして将来の可能性について議論する.
- 確立された技術と革新的な細胞培養技術の概要を提供するためです.
主な方法:
- 商業出版物と研究出版物の文献レビュー.
- 確立された大規模細胞培養プロセスの分析.
- 最近のイノベーションとその潜在的な影響の探求.
主要な成果:
- 商業的な大規模細胞培養は,主に哺乳類の細胞を使用しています.
- 主な課題には,プロセスのスケーラビリティ,コスト効率,規制遵守などがあります.
- 新興のイノベーションは,効率の向上と新しいアプリケーションの有望さを示しています.
結論:
- 大規模な細胞培養は,バイオテクノロジーの重要な分野であり続けています.
- 現在の課題に取り組むことは,将来の可能性を解放する鍵です.
- 継続的なイノベーションにより,セルベースの製造の進歩が期待されます.
関連する概念動画
01:21Cell Culture
Most vertebrate cells grow in vitro attached to a substrate as a monolayer, called adherent cultures. The flasks and plates used to grow cells are chemically treated to facilitate cell attachment. However, a few cell types, such as hematopoietic cells, can grow in a suspension. In contrast to adherent cultures, suspension cultures can grow in non-treated cultureware using magnetic stirrers or spinner flasks to agitate the culture media
01:16Cell Lines
A cell line is a population of cells grown in vitro that can be subcultured over several generations. Normal cells cease to divide after a certain number of cell divisions, a process known as replicative senescence. This number, called the Hayflick limit, was conceptualized by Leonard Hayflick in 1961 when he observed that fetal cells grown in culture could only divide 40-60 times. This limit is due to the shortening of the telomeres during each round of cell division, preventing cell division...
01:29Bioreactor Design and Operational System
Bioreactors are engineered vessels designed to cultivate microorganisms under controlled conditions for industrial bioprocessing. They maintain sterility and allow precise regulation of pH, temperature, oxygen, and nutrient levels to optimize microbial growth and metabolite production. Bioreactors range from small laboratory units of 1 liter to industrial systems holding up to 500,000 liters, though only about 75% of their volume is actively used for fermentation. The remaining headspace...
01:23Fed-Batch Culture
Fed-batch culture is a widely used bioprocessing strategy combining aspects of batch culture with controlled substrate feeding to optimize cell growth and product formation. In this semi-closed system, nutrients are strategically added during fermentation, while the accumulated products and biomass remain within the bioreactor until the end of the operation. This controlled addition of substrates allows for better management of growth kinetics, nutrient limitation, and metabolite...
01:14Scale-Up Processes
The scale-up of microbial fermentation processes is essential in industrial biotechnology, allowing the transition from laboratory-scale experiments to commercial-scale production while aiming to maintain product yield and quality. This process requires meticulous adjustment of equipment design, process parameters, and contamination control strategies to accommodate increasing culture volumes.At the laboratory scale, cultures are typically maintained in 1 to 10-liter glass or autoclavable...
01:27Upstream Processing
Upstream processing represents a critical phase in biomanufacturing, wherein biological systems such as microorganisms, mammalian cells, or insect cells are cultivated to produce therapeutic proteins, vaccines, enzymes, or other biologically derived products. This phase encompasses all steps from the selection and genetic manipulation of the production organism to the cultivation of cells in bioreactors under tightly controlled environmental conditions.Host Selection and Genetic OptimizationThe...