ブラウンマクロアルゲの砂糖から合成酵母プラットフォームによる効率的なエタノール生産
Maria Enquist-Newman1, Ann Marie E Faust1, Daniel D Bravo1
11] Bio Architecture Lab Inc., 604 Bancroft Way, Suite A, Berkeley, California 94710, USA [2].
Nature
|December 3, 2013
まとめ
茶色のマクロ藻は,持続可能なバイオ燃料に使用することができます. 研究者らは,酵母を設計して藻類からアルギナートとマニトールを効率的に代謝させ,従来の原料と比較して重要なエタノール生産を可能にしました.
科学分野:
- バイオテクノロジー バイオテクノロジー
- 再生可能エネルギーの再生可能エネルギー
- 海の資源 海の資源 海の資源
背景:
- 食料と燃料の需要の増加により,持続可能な原料が必要になります.
- 茶色のマクロ藻は,有望な,未充分に利用された資源を提供します.
- 現在,Saccharomyces cerevisiaeのような産業用微生物には,アルギナットとマニトールの利用のための効率的な経路がない.
研究 の 目的:
- ブラウンマクロ藻の原料の効率的な利用のためにSaccharomyces cerevisiaeを設計する.
- アルギナットとマニトールから商業規模でバイオ燃料を生産できるようにする.
- 主要な藻類の糖質のための新しいトランスポーターを発見し,実装する.
主な方法:
- アステロマイセス・クルシアタス (Asteromyces cruciatus) の新しいアルギナートモノマー (DEHU) トランスポーターを発見し,特徴づけました.
- ゲノム的にDEHUトランスポーター遺伝子とバクテリアのアルギナート分解遺伝子をS. cerevisiaeに統合しました.
- S. cerevisiae. のネイティブマニトール分解経路を非調節した.
- 合成株を無酸素成長と発酵に適応した.
主要な成果:
- エンジニアリングされたS. cerevisiaeはDEHUとマンニトールの両方を効率的に代謝しました.
- これらの糖から,無酸素条件下で,著しいエタノール発酵を達成した.
- 取得したエタノールの位数は4.6% (v/v) で,理論上の最大値の83%まで生産します.
結論:
- バイオ燃料の総合的な原料として茶色マクロ藻を使用する可能性を実証しました.
- 藻類の砂糖からバイオ燃料と再生可能な化学物質を生産するためのプラットフォームを設立しました.
- 藻類ベースの原料は,従来の耕地ベースの資源と競争することができます.
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