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Receptor-mediated Endocytosis01:38

Receptor-mediated Endocytosis

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Receptor-mediated Endocytosis01:20

Receptor-mediated Endocytosis

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Receptor-mediated endocytosis is when bulk amounts of specific molecules are imported into a cell after binding to cell surface receptors. The molecules bound to these receptors are taken into the cell through inward folding of the cell surface membrane, which is eventually pinched off into a vesicle within the cell. Structural proteins, such as clathrin, coat the budding vesicle.
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One well-characterized example of receptor-mediated endocytosis is the...
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Membrane-enclosed structures called vesicles transport proteins and lipids across the cell. The vesicles derive their cargo from the plasma membrane, Golgi, ER, or endosome. Coated vesicles are spherical, protein-coated carriers with a 50–100 nm diameter that mediate bidirectional transport between the ER and the Golgi. The distribution of proteins between the ER and Golgi complex is dynamic and is maintained by different coated vesicles. Their formation is driven by the assembly of...
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Vesicle budding is orchestrated by distinct cytosolic proteins such as adaptor proteins, coat proteins, and GTPases. To initiate vesicle budding, membrane-bending proteins containing crescent-shaped BAR domains bind to the lipid heads in the bilayer and distort the membrane to form a protein-coated vesicle bud. Adaptors proteins such as AP2 for clathrin-coated vesicles can nucleate on the deformed membrane. Finally, coat proteins such as clathrin or COPI and COPII assemble into a coat forming...
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Clathrin-coated vesicles use endocytosis to transport receptors and lysosomal hydrolases from the Golgi to the lysosome in the late secretory pathway. Clathrin-mediated endocytosis was the first described endocytic process, and Clathrin-coated vesicles remain one of the most well-studied transport vesicles. The molecular machinery that generates clathrin-coated vesicles comprises over 50 proteins that precisely coordinate vesicle formation. Cell surface receptors concentrated in indented sites...
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半覆いジャヌス粒子が細胞に侵入する方法

Yuan Gao1, Yan Yu

  • 1Department of Chemistry, Indiana University , Bloomington, Indiana 47405, United States.

Journal of the American Chemical Society
|December 7, 2013
PubMed
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この要約は機械生成です。

ジャヌス粒子は,ユニークな非対称的なデザインで,細胞の吸収を明確な3段階のプロセスで導く. この研究は,これらの微粒子のリガンド分布が,エンドサイトーシス中の細胞膜ダイナミクスにどのように影響するか明らかにしています.

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科学分野:

  • バイオマテリアル科学 バイオマテリアル科学
  • 細胞生物学 細胞生物学
  • ナノテクノロジー ナノテクノロジー

背景:

  • ジャヌス粒子は,先進的な生物医学アプリケーションのために機能的非対称性を提供します.
  • ジャヌス粒子と細胞の相互作用,特に細胞の吸収を理解することは極めて重要ですが,完全に解明されていません.
  • 均質な粒子には,ジャヌス系で見られる方向制御が欠けている.

研究 の 目的:

  • ジャヌス微粒子の非対称リガンド分布が受容体媒介吸収中の細胞膜ダイナミクスにどのように影響するか調査する.
  • ジャヌス粒子の吸収機構と同質粒子の吸収機構を比較する.
  • 粒子-細胞相互作用を決定するジャヌス原理の役割を明らかにする.

主な方法:

  • 粒子-細胞の相互作用を視覚化するために,生細胞の光成像を用いた.
  • 吸収中の膜ダイナミクスの単粒子レベルの定量化が行われました.
  • 受容体媒介性エンドサイトーシスの経路は,ジャヌス粒子幾何学の文脈で研究されました.

主要な成果:

  • ジャヌス粒子の吸収には,独特の3段階の内細胞プロセスが含まれます: 膜カップ形成,ジャヌスインターフェイスで停滞,およびリガンドのない半球の突起.
  • 非対称なリガンド分布は,均質な粒子の吸収と異なる膜ダイナミクスを著しく左右する.
  • リガンドの空間的表現と一時膜のダイナミクスとの間には直接的な相関が観察されました.

結論:

  • ジャヌス粒子の非対称性は,均質な粒子と比較して,細胞の吸収メカニズムを根本的に変化させます.
  • ジャヌス粒子のリガンドの空間的配置は,細胞の相互作用を正確に制御するメカニズムを提供します.
  • この研究は,生物医学アプリケーションにおける標的細胞相互作用のエンジニアリングのためのジャヌス原理の可能性を強調しています.