関連する実験動画

Updated: May 4, 2026

Pooled CRISPR-Based Genetic Screens in Mammalian Cells
09:05

Pooled CRISPR-Based Genetic Screens in Mammalian Cells

Published on: September 4, 2019

22.0K

ヒト細胞におけるゲノムスケールCRISPR-Cas9ノックアウトスクリーニング

Ophir Shalem1,2, Neville E Sanjana1,2, Ella Hartenian1

  • 1Broad Institute of MIT and Harvard, 7 Cambridge Center, MA 02142, USA.

Science (New York, N.Y.)
|December 17, 2013
PubMed
まとめ

この研究では,効率的な遺伝子機能スクリーニングのためのゲノム規模のCRISPR-Cas9ノックアウト (GeCKO) ライブラリを導入しています. この技術は,ヒト細胞における重要な遺伝子と薬剤耐性因子を成功裏に特定し,生物学的発見のための潜在能力を示しました.

さらに関連する動画

Cell Surface Receptor Identification Using Genome-Scale CRISPR/Cas9 Genetic Screens08:49

Cell Surface Receptor Identification Using Genome-Scale CRISPR/Cas9 Genetic Screens

Published on: June 6, 2020

15.2K
Using a Fluorescent PCR-capillary Gel Electrophoresis Technique to Genotype CRISPR/Cas9-mediated Knockout Mutants in a High-throughput Format08:25

Using a Fluorescent PCR-capillary Gel Electrophoresis Technique to Genotype CRISPR/Cas9-mediated Knockout Mutants in a High-throughput Format

Published on: April 8, 2017

13.1K

関連する概念動画

CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
3.2K