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関連する概念動画

DNA Helicases00:55

DNA Helicases

24.9K
DNA unwinding helicase enzymes are a type of motor protein. Motor proteins can translocate along filaments or polymers using energy generated from ATP hydrolysis. Helicases are involved in all the important cellular processes where DNA unwinding is required, such as DNA replication, repair, recombination, and transcription. They are present in all living organisms, but vary in their structure, function, and mechanism of action. For example, in prokaryotes, DnaB helicase binds and translocates...
24.9K
Homologous Recombination02:31

Homologous Recombination

65.5K
The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
65.5K
DNA Topoisomerases02:02

DNA Topoisomerases

37.5K
Topoisomerases are enzymes that relax overwound DNA molecules during various cell processes, including DNA replication and transcription. These enzymes regulate positive and negative DNA supercoiling without changing the nucleotide sequence. DNA overwinding in a clockwise direction results in positively supercoiled DNA, whereas underwinding in a counterclockwise direction produces negatively supercoiled DNA.
Types and Mechanism of action
Topoisomerases are divided into two main types. ...
37.5K
Single-Strand DNA Binding Proteins01:03

Single-Strand DNA Binding Proteins

17.2K
For successful DNA replication, the unwinding of double-stranded DNA must be accompanied by stabilization and protection of the separated single strands of the DNA. This crucial task is performed by single-strand DNA-binding (SSB) proteins. They bind to the DNA in a sequence-independent manner, which means that the nitrogenous bases of the DNA need not be present in a specific order for binding of SSB proteins to it. The binding of SSB proteins straightens single-stranded DNA (ssDNA) and makes...
17.2K
The DNA Replication Fork01:02

The DNA Replication Fork

43.1K
An organism’s genome needs to be duplicated in an efficient and error-free manner for its growth and survival. The replication fork is a Y-shaped active region where two strands of DNA are separated and replicated continuously. The coupling of DNA unzipping and complementary strand synthesis is a characteristic feature of a replication fork.   Organisms with small circular DNA, such as E. coli, often have a single origin of replication; therefore, they have only two replication...
43.1K
The DNA Replication Fork01:02

The DNA Replication Fork

20.3K
No description available
20.3K

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関連する実験動画

Updated: Mar 30, 2026

Single-Molecule Real-Time Visualization of DNA Unwinding by CMG Helicase
07:37

Single-Molecule Real-Time Visualization of DNA Unwinding by CMG Helicase

Published on: September 27, 2024

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開発的に規制された活動で,RNA複合体を解き放つ.

B L Bass, H Weintraub

    Cell
    |February 27, 1987
    PubMed
    まとめ

    Xenopus胚におけるアンチセンセスのRNA技術は,RNA:RNAハイブリッドの解き放たれ活動により,一時的なものである. この活動は,胚形成を通して卵細胞から存在し,遺伝子調節のための安定したハイブリッド形成を妨げます.

    科学分野:

    • 発達生物学 発達生物学について
    • 分子生物学は分子生物学である.
    • RNA 生物学 RNA 生物学

    背景:

    • アンティセンスRNA技術は,遺伝子調節のためのツールです.
    • RNA:RNAハイブリッドは,遺伝子調節における重要な中間物質である.
    • これらのハイブリッドの安定性は,反感覚RNAの有効性にとって極めて重要です.

    研究 の 目的:

    • 開発中のXenopus胚における感覚:反感覚RNAハイブリッドの安定性を調査する.
    • このモデル生物におけるアンチセンセスのRNA技術の有効性を制限する要因を特定する.

    主な方法:

    • Xenopusの胚でアンチセンセスのRNA技術を活用した.
    • 分析されたRNA:RNAハイブリッドの形成と安定性.
    • Xenopusの卵細胞と胚からのS100抽出物における特徴的なRNA解き放出活性.

    主要な成果:

    • Sense:antisense RNAハイブリッドが形成されたが,Xenopus胚では一時的であった.
    • RNA:RNAハイブリッドの解き放たれ活動は卵細胞で検出され,成熟と胚形成の間に増加しました.
    • この活動はS100抽出物で特徴付けられ,複製RNAに特異的であることが示されました.

    さらに関連する動画

    Artificial RNA Polymerase II Elongation Complexes for Dissecting Co-transcriptional RNA Processing Events
    10:59

    Artificial RNA Polymerase II Elongation Complexes for Dissecting Co-transcriptional RNA Processing Events

    Published on: May 13, 2019

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    A Fluorescence-based Exonuclease Assay to Characterize DmWRNexo, Orthologue of Human Progeroid WRN Exonuclease, and Its Application to Other Nucleases
    06:10

    A Fluorescence-based Exonuclease Assay to Characterize DmWRNexo, Orthologue of Human Progeroid WRN Exonuclease, and Its Application to Other Nucleases

    Published on: December 23, 2013

    5.7K

    関連する実験動画

    Last Updated: Mar 30, 2026

    Single-Molecule Real-Time Visualization of DNA Unwinding by CMG Helicase
    07:37

    Single-Molecule Real-Time Visualization of DNA Unwinding by CMG Helicase

    Published on: September 27, 2024

    2.7K
    Artificial RNA Polymerase II Elongation Complexes for Dissecting Co-transcriptional RNA Processing Events
    10:59

    Artificial RNA Polymerase II Elongation Complexes for Dissecting Co-transcriptional RNA Processing Events

    Published on: May 13, 2019

    10.3K
    A Fluorescence-based Exonuclease Assay to Characterize DmWRNexo, Orthologue of Human Progeroid WRN Exonuclease, and Its Application to Other Nucleases
    06:10

    A Fluorescence-based Exonuclease Assay to Characterize DmWRNexo, Orthologue of Human Progeroid WRN Exonuclease, and Its Application to Other Nucleases

    Published on: December 23, 2013

    5.7K

    結論:

    • 強力なRNA:RNAハイブリッド解き放つ活動は,Xenopus.のアンチセンセスのRNAハイブリッドの安定性を制限しています.
    • この活動は発達的に調節され,オオゲネシスから胚形成まで存在します.
    • この発見は,Xenopusの発達初期に直接的なアンチセンセスのRNA戦略を適用する際の課題を示唆している.