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Updated: May 4, 2026

Monitoring the Reductive and Oxidative Half-Reactions of a Flavin-Dependent Monooxygenase using Stopped-Flow Spectrophotometry
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Monitoring the Reductive and Oxidative Half-Reactions of a Flavin-Dependent Monooxygenase using Stopped-Flow Spectrophotometry

Published on: March 18, 2012

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菌類のポリサッカリドモノオキシゲナーゼにおける地域選択的水酸化の決定因子

Van V Vu1, William T Beeson, Christopher M Phillips

  • 1Department of Chemistry, The Scripps Research Institute , La Jolla, California 92037, United States.

Journal of the American Chemical Society
|December 20, 2013
PubMed
まとめ

菌類のポリサッカリドモノオキシゲネーゼ (PMO) は,固有の系統遺伝グループを示し,グリコシド結合の特定の水酸化を可能にします. この研究は,よりよい酵素理解のために,PMOの構造をその化学的地域選択性と関連付けています.

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A significant aspect of hydroboration–oxidation is the regio- and stereochemical outcome of the reaction.
Hydroboration proceeds in a concerted fashion with the attack of borane on the π bond, giving a cyclic four-centered transition state. The –BH2 group is bonded to the less substituted carbon and –H to the more substituted carbon. The concerted nature requires the simultaneous addition of –H and –BH2 across the same face of the alkene giving syn...
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If a set of reactants can yield multiple constitutional isomers, but one of the isomers is obtained as the major product, the reaction is said to be regioselective. In such reactions, bond formation or breaking is favored at one reaction site over others.
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