関連する実験動画

Updated: Aug 11, 2026

Methods to Discover Alternative Promoter Usage and Transcriptional Regulation of Murine Bcrp1
11:02

Methods to Discover Alternative Promoter Usage and Transcriptional Regulation of Murine Bcrp1

Published on: May 27, 2016

哺乳類DNAの潜在的な遺伝子配列を検出するために制限酵素の使用

S Lindsay, A P Bird

    Nature
    |May 3, 1987
    PubMed
    まとめ

    研究者らは,クローンDNAの遺伝子豊富な領域 (HTF島) を識別する方法を開発した. このテクニックは,脊椎動物のゲノム内の遺伝子機能を理解するために不可欠な転写開始部位を特定するのに役立ちます.

    さらに関連する動画

    Single Cell Multiplex Reverse Transcription Polymerase Chain Reaction After Patch-clamp10:44

    Single Cell Multiplex Reverse Transcription Polymerase Chain Reaction After Patch-clamp

    Published on: June 20, 2018

    High-throughput Identification of Gene Regulatory Sequences Using Next-generation Sequencing of Circular Chromosome Conformation Capture (4C-seq)09:06

    High-throughput Identification of Gene Regulatory Sequences Using Next-generation Sequencing of Circular Chromosome Conformation Capture (4C-seq)

    Published on: October 5, 2018

    関連する概念動画

    In-vitro Mutagenesis01:16

    In-vitro Mutagenesis

    To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.
    Conservative Site-specific Recombination and Phase Variation02:53

    Conservative Site-specific Recombination and Phase Variation

    Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
    The recognition sites for Cre recombinase called LoxP...
    DNA Isolation01:24

    DNA Isolation

    DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...
    Sanger Sequencing01:57

    Sanger Sequencing

    DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
    Reporter Genes02:11

    Reporter Genes

    Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
    Commonly used reporter...
    Restriction Enzymes01:11

    Restriction Enzymes

    Restriction enzymes are bacterial enzymes used to cut DNA in a sequence-specific manner. To cleave DNA, they bind to specific palindromic sequences called restriction sites. Such palindromic DNA sequences or inverted repeats are commonly found in regions of functional significance, such as the origin of replication, gene operator sites, and regions containing transcription termination signals.
    The host bacteria protect their own genomic DNA from these enzymes by methylating these sites. Some...