関連する実験動画
Updated: Jul 19, 2026

06:59
A Reverse Genetic Approach to Test Functional Redundancy During Embryogenesis
Published on: August 11, 2010
まとめ
ドロソフィラの発達における無翼遺伝子は,細胞の協力に不可欠である. Antisense RNAは,特定のトランスクリプトが翼のない機能を実行することを確認し,マウス int-1のドロソフィラ同型体として識別します.
科学分野:
- 発達生物学 発達生物学について
- 遺伝学 遺伝学とは
- 分子生物学は分子生物学である.
背景:
- ドロソフィラ・メラノガスターの無翼遺伝子は,発達中の細胞協力に役割を果たすことが知られている.
- 正確な分子メカニズムと,翼のない機能に起因する特定のトランスクリプトは,完全に解明されていません.
研究 の 目的:
- 翼のない遺伝子の役割を実行する特定の3kbのトランスクリプトの機能を確認するために.
- 翼のない遺伝子の同一性をマウスのint-1遺伝子のクローンドロソフィラ同種と確認する.
主な方法:
- 3kBのトランスクリプトから得られたアンチセンセスのRNAを野生型のドロソフィラ卵に注入する.
- 孤立した無翼のcDNAの配列解析.
- 孤立した無翼のcDNA配列とマウスint-1 (Dint-1) のドロソフィラ同型との比較.
主要な成果:
- 3kBのトランスクリプトのアンチセンセスのRNAを注入すると,野生型の胚で翼のない変異体のフェノコピーを成功裏に生成しました.
- 孤立した部分的な無翼のcDNA配列は,Dint-1配列の一部と同一であることが判明しました.
- マウス int-1 (Dint-1) のドロソフィラ同種は,翼のない遺伝子と同一であることが確認されました.
結論:
- 3kbのトランスクリプトは,ドロソフィラの発達における無翼遺伝子の機能に決定的に責任があります.
- 翼のない遺伝子はマウスint-1遺伝子のドロソフィラ同型であり,保存された発達経路を示しています.
関連する概念動画
RNA Interference
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
In-vitro Mutagenesis
To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.
RNA Interference
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
siRNA - Small Interfering RNAs
Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the ATP-dependent...
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the ATP-dependent...
piRNA - Piwi-interacting RNAs
PIWI-interacting RNAs, or piRNAs, are the most abundant short non-coding RNAs. More than 20,000 genes have been found in humans that code for piRNAs while only 2000 genes have been found for miRNAs. piRNAs can act at the transcriptional and post-transcriptional levels and have a vital role in silencing transposable elements present in germ cells. They are also involved in epigenetic silencing and activation. Previously, they were thought to function only in germ cells but new evidence suggests...
Experimental RNAi
RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...

