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単細胞RNA-seqは,哺乳類の細胞におけるダイナミックでランダムなモノアレル基因発現を明らかにしています
Qiaolin Deng1, Daniel Ramsköld, Björn Reinius
1Ludwig Institute for Cancer Research, Box 240, 171 77 Stockholm, Sweden.
Science (New York, N.Y.)
|January 11, 2014
まとめ
哺乳類の細胞は,ランダムな単アルレ性遺伝子の発現が豊富で,遺伝子の複製のうちの2つのうちの1つだけが活発である. これは,成熟した組織でも,個々の細胞間で独立して動的に発生します.
科学分野:
- ゲノミクスゲノミクスとは
- 発達生物学 発達生物学について
- エピジェネティクス エピジェネティクス
背景:
- ディプロイド細胞は,通常,両方の遺伝子アレルの発現を示します.
- 単細胞における全ゲノムにわたるアレル発現パターンは,ほとんど特徴づけられていないままである.
研究 の 目的:
- 個々のマウスの胚細胞における全ゲノムにわたるアレル発現パターンを調査する.
- モノアレル基因発現の流行と動態を決定する.
主な方法:
- マウスのミックス遺伝子背景 (CAST/EiJ × C57BL/6J) のプリインプラント胚の単細胞分析.
- オートソームおよびX染色体のアレル発現のグローバル評価.
主要な成果:
- 自体遺伝子の豊富なモノアレル発現 (12-24%) が観察されました.
- アレルの発現は,密接に関連した細胞間で独立,ランダム,およびダイナミックであることが判明しました.
- 父のX染色体のデノボ不活性化が実証されました.
- 同じようなモノアレル発現パターンは,成熟した細胞でも検出されました.
結論:
- 独立かつストキャスティックなアレル転写は,哺乳類の細胞における豊富なランダムなモノアレル発現を誘導する.
- アレル発現は,隣接する細胞間でも著しく変化するダイナミックなプロセスです.
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