関連する実験動画
Updated: Aug 7, 2026

10:31
Real-time Live Imaging of T-cell Signaling Complex Formation
Published on: June 23, 2013
中間T抗原とpp60c-srcで複合した81kdタンパク質:フォスファディチルニノシトールキナーゼの可能性
Cell
|September 25, 1987
まとめ
研究者らは,中間T抗原とpp60c-srcの複合体の中で,新しいタンパク質であるp81を特定しました. このタンパク質,p81はチロシン・フォスフォリレーテッド・フォスファディチルニノシトールキナーゼであり,ポリオマウイルスの変異に不可欠です.
科学分野:
- 分子生物学は分子生物学である.
- ウイルス学 ウイルス学 ウイルス学
- セルラー・シグナリング
背景:
- 中部T抗原は,細胞シグナル伝達における重要なタンパク質であるpp60c-srcと複合体を形成する.
- この複合体の正確な構成と機能,特にウイルスの変異において,さらなる解明が必要である.
研究 の 目的:
- 中部T抗原-pp60c-src複合体の追加の成分を特定し,特徴づけること.
- この複合体に関連した酵素活性と,ポリオマウイルス変異におけるその役割を調査する.
主な方法:
- 中間T抗原とpp60c-srcに対する抗体を用いた免疫プレシピテーション.
- タンパク質のリン酸化を検出するために,イムノプレシピテートに対するキナーゼアッセイ.
- 32Pで標識された細胞溶解物の分析.
- 免疫複合体におけるフォスファディチルニノシトールキナーゼの活性を測定する.
主要な成果:
- 81 kDa (p81) の新しいタンパク質が,中位T抗原-pp60c-src複合体の安定成分として特定されました.
- p81は,タイロシン残基のみでリン酸化される.
- リン酸化p81の存在は,中間T抗原の変異変異体と相関している.
- フォスファディチルニノシトールキナーゼの活性性は,p81.1を含む複合体においてのみ発見されました.
結論:
- p81はフォスファティディル・イノシトール・キナーゼである.
- p81は中部T抗原-pp60c-src複合体の重要な成分であり,フォスファティジル・イノシトールキナーゼの活性を媒介する.
- 中間T抗原とpp60c-srcとのp81の相互作用は,ポリオマウイルス媒介の細胞変異に不可欠です.
関連する概念動画
Phosphoinositides and PIPs
Phosphoinositides are a group of phospholipids containing a glycerol backbone with two fatty acid chains and a phosphate attached to a myoinositol sugar ring. The inositol head group extends into the cytoplasm, where it is modified by adding phosphate groups to form phosphatidylinositol phosphates or PIPs.
Different phosphoinositides are synthesized and recruited on the cytosolic face of the plasma membrane. The localization of specific phosphoinositides concentrated in separate membrane...
Different phosphoinositides are synthesized and recruited on the cytosolic face of the plasma membrane. The localization of specific phosphoinositides concentrated in separate membrane...
Anaphase Promoting Complex
The stepwise destruction of specific proteins is necessary for the progression and completion of the cell cycle. Such proteins are ubiquitinated by ubiquitin ligases and then subsequently destroyed by the proteasome. The SCF (Skp1/Cullin/F-box) and the anaphase-promoting complex (APC) are two important ubiquitin ligases involved in cell cycle progression. While SCF is active throughout the cell cycle, APC gets activated during metaphase to anaphase transition. Cdc20 or Cdh1 binds to APC and...
Assembly of Signaling Complexes
Multiprotein signaling complexes are formed in a dynamic process involving protein-protein interactions at the cytoplasmic domain of transmembrane receptors or enzymatic and non-enzymatic proteins associated with the receptor. These complexes ensure the activation and propagation of intracellular signals that regulate cell functions.
Interaction domains in cell signaling
Interaction domains recognize exposed features of their binding partners containing post-translationally modified sequences,...
Interaction domains in cell signaling
Interaction domains recognize exposed features of their binding partners containing post-translationally modified sequences,...
Receptor Tyrosine Kinases
Receptor tyrosine kinases or RTKs are membrane-bound receptors that phosphorylate specific tyrosine on protein substrates. RTKs regulate cellular growth, differentiation, survival, and migration. They contain an extracellular ligand binding domain, a transmembrane domain, and a cytosolic tail with intrinsic kinase activity. Several extracellular signaling molecules activate RTKs in one or more ways and relay the signal downstream. Ligands such as platelet-derived growth factor (PDGF) or...
MAPK Signaling Cascades
Mitogen-activated protein kinase, or MAPK pathway, activates three sequential kinases to regulate cellular responses such as proliferation, differentiation, survival, and apoptosis. The canonical MAPK pathway starts with a mitogen or growth factor binding to an RTK. The activated RTKs stimulate Ras, which recruits Raf or MAP3 Kinase (MAPKKK), the first kinase of the MAPK signaling cascade. Raf further phosphorylates and activates MEK or MAP2 Kinases (MAPKK), which in turn phosphorylates MAP...
PI3K/mTOR/AKT Signaling Pathway
The mammalian target of rapamycin (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1 (mTORC1) and mTOR complex 2 (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast, mTORC2 consists of a rapamycin-insensitive companion...

