細胞外ATPに対する植物受容体の特定
Jeongmin Choi1, Kiwamu Tanaka, Yangrong Cao
1Divisions of Biochemistry and Plant Sciences, Christopher S. Bond Life Sciences Center, University of Missouri, Columbia, MO 65211, USA.
Science (New York, N.Y.)
|January 18, 2014
まとめ
植物は,成長とストレス反応のために細胞外アデノシン5'-トリフォスファート (ATP) を利用する. 研究者らは,DORN1タンパク質をアラビドプシスの細胞外ATPの主要な受容体として特定し,植物シグナル伝達とストレス抵抗に不可欠である.
科学分野:
- 植物生物学 植物生物学
- 分子シグナリング
- バイオケミストリー バイオケミストリー
背景:
- 細胞外アデノシン5トリホスファート (ATP) は,哺乳類における重要なシグナル伝達分子であり,P2型純素受容体を通して作用する.
- 植物には直接的なATP受容体がないが,成長,発達,ストレス反応のために細胞外ATPを利用する.
研究 の 目的:
- 植物が細胞外ATPを感知するために使用する分子機構を特定する.
- 植物反応における特定された受容体の役割を特徴づける.
主な方法:
- アラビドプシスのATP無敏感変異体を特定するための遺伝子スクリーニング.
- 特定されたタンパク質のATP結合親和性を決定する生化学分析.
- ATPと傷害に対する突然変異の反応を評価するための生理学的分析.
主要な成果:
- レクチン受容体キナーゼI.9 (DORN1) に欠陥があるATP無感変異体dorn1の特定.
- DORN1は高親和性ATP結合 (Kd = 45.7 ± 3.1 nM) を示しています.
- DORN1はATP誘発カルシウムシグナル伝達,MAPK活性化,遺伝子発現に不可欠であり,子宮外発現は傷口反応を高める.
結論:
- DORN1は,アラビドプシスの細胞外ATPの高親和受容体として機能する.
- DORN1は,特にストレス耐性において,細胞外ATPに対する植物の反応を媒介するために重要である.
- この発見は,植物-細胞外ATPシグナル伝達を理解するための分子基盤を提供します.
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