乳がん患者からのモノサイトにおけるレトロウイルスの性質を持つ粒子
A M Al-Sumidaie1, S J Leinster, C A Hart
1University Department of Surgery, University of Liverpool.
Lancet (London, England)
|January 2, 1988
まとめ
研究者らは,乳がん患者の単細胞にレトロウイルスに似た物質を発見し,逆転写酵素の活性と特定の粒子の形態学によって証明されました. これは,乳がんとレトロウイルスの潜在的な関連性を示唆しており,その役割に関するさらなる調査を正当化しています.
科学分野:
- 腫瘍学 腫瘍学
- ウイルス学 ウイルス学 ウイルス学
- 免疫学 免疫学とは
背景:
- モノサイトは,様々な癌に関与する重要な免疫細胞です.
- レトロウイルスは,細胞のプロセスと疾患の発症に影響を及ぼすことが知られている.
研究 の 目的:
- 乳がん患者の単細胞にレトロウイルス剤の存在を調査する.
- これらの物質の性質と乳がんとの関連性を特徴づける.
主な方法:
- リバーストランスクリプトーゼ (RT) 活性に対するモノサイト培養と細胞フリー培養媒介 (CFCM) 分析.
- 浮気密度離心分離と非イオン性洗浄剤による処理により,粒子を特徴付けます.
- ウイルス形態の視覚化のためのネガティブステイン電子顕微鏡.
主要な成果:
- 乳がん患者のCFCMにおけるRT活動の高頻度 (97%),対照群では11%であった.
- 洗剤に敏感なレトロウイルスのような粒子 (密度1.165-1.18g/ml) と関連するRT活動.
- 電子顕微鏡では,CFCMのネズミの乳腺腫瘍ウイルスと患者からのマクロファージに似た包装された粒子を明らかにしました.
結論:
- 強力な証拠は,乳がん患者の単細胞内にレトロウイルスが存在することを示唆しています.
- 乳がんの病理生理学におけるこのレトロウイルスの役割については,さらなる解明が必要である.
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