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Updated: May 3, 2026

Anaerobic Protein Purification and Kinetic Analysis via Oxygen Electrode for Studying DesB Dioxygenase Activity and Inhibition
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Anaerobic Protein Purification and Kinetic Analysis via Oxygen Electrode for Studying DesB Dioxygenase Activity and Inhibition

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調節成分によって媒介される溶性メタンモノオキシゲナーゼの活性部位への基板アクセスの二酸化鉄酸化状態制御

Weixue Wang1, Stephen J Lippard

  • 1Department of Chemistry, Massachusetts Institute of Technology , Cambridge, Massachusetts 02139, United States.

Journal of the American Chemical Society
|January 31, 2014
PubMed
まとめ

溶性メタンモノオキシゲナーゼのユニークなN端尾.

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Oxymercuration–reduction of alkenes is one of the major reactions converting alkenes to alcohols. It involves the hydration of alkenes with mercuric acetate in a mixture of tetrahydrofuran and water, forming an organomercury adduct. This is followed by a demercuration step in which the adduct is reduced to an alcohol using sodium borohydride.
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