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DNA Helicases00:55

DNA Helicases

19.4K
DNA unwinding helicase enzymes are a type of motor protein. Motor proteins can translocate along filaments or polymers using energy generated from ATP hydrolysis. Helicases are involved in all the important cellular processes where DNA unwinding is required, such as DNA replication, repair, recombination, and transcription. They are present in all living organisms, but vary in their structure, function, and mechanism of action. For example, in prokaryotes, DnaB helicase binds and translocates...
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Golgi Matrix Proteins01:12

Golgi Matrix Proteins

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Golgi matrix proteins are a group of highly dynamic proteins that maintain the stacked structure of Golgi. These proteins adapt to rapid morphological changes of the Golgi during the cell cycle. During cell division, mild proteolysis removes these connections resulting in Golgi unstacking. In The daughter cells, these proteins help reassemble the unstacked Golgi.
One of the first identified Golgi matrix proteins was GM130, a rod-like protein located in the cis-Golgi. Subsequently, many Golgi...
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DNA Topoisomerases02:02

DNA Topoisomerases

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Topoisomerases are enzymes that relax overwound DNA molecules during various cell processes, including DNA replication and transcription. These enzymes regulate positive and negative DNA supercoiling without changing the nucleotide sequence. DNA overwinding in a clockwise direction results in positively supercoiled DNA, whereas underwinding in a counterclockwise direction produces negatively supercoiled DNA.
Types and Mechanism of action
Topoisomerases are divided into two main types. ...
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The DNA Replication Fork01:02

The DNA Replication Fork

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An organism’s genome needs to be duplicated in an efficient and error-free manner for its growth and survival. The replication fork is a Y-shaped active region where two strands of DNA are separated and replicated continuously. The coupling of DNA unzipping and complementary strand synthesis is a characteristic feature of a replication fork.   Organisms with small circular DNA, such as E. coli, often have a single origin of replication; therefore, they have only two replication...
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The DNA Replication Fork01:02

The DNA Replication Fork

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The DNA Helix01:07

The DNA Helix

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Deoxyribonucleic acid, or DNA, is the genetic material responsible for passing traits from generation to generation in all organisms and most viruses. DNA is composed of two strands of nucleotides that wind around each other to form a spring-like structure called a double helix. However, the double helix is not perfectly symmetrical. Instead, there are regularly occurring grooves in the structure. The major groove occurs where the sugar-phosphate backbones are relatively far apart. This space...
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Analyzing and Building Nucleic Acid Structures with 3DNA
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Analyzing and Building Nucleic Acid Structures with 3DNA

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ゴルギはDNAの痛みを感じています.

Marco Foiani1, Jiri Bartek2

  • 1IFOM (Fondazione Istituto FIRC di Oncologia Molecolare), Via Adamello 16, 20139 Milan, Italy; Università degli Studi di Milano, Milan 20122, Italy.

Cell
|February 4, 2014
PubMed
まとめ
この要約は機械生成です。

DNAの損傷により,ゴルジ装置が分散し,膀輸送が停止する. これは,DNA-PK媒介によるGOLPH3のリン酸化を経由して発生し,DNA損傷反応とGolgi調節を結びつける.

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DNA Tension Probes to Map the Transient Piconewton Receptor Forces by Immune Cells
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DNA Tension Probes to Map the Transient Piconewton Receptor Forces by Immune Cells
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科学分野:

  • 細胞生物学 細胞生物学
  • 分子生物学は分子生物学である.
  • バイオケミストリー バイオケミストリー

背景:

  • タンパク質の改変と輸送に不可欠なゴルギ器官は,F-アクチンとGOLPH3.3を通じてその構造を維持しています.
  • ゴルギの整合性は,細胞の機能と刺激への反応に不可欠です.

研究 の 目的:

  • ゴルギ装置の構造と機能に対するDNA損傷の影響を調査する.
  • DNA損傷反応とゴルギの調節を結びつける分子メカニズムを解明する.

主な方法:

  • この研究では,ゴルギの形態と膀輸送を評価するための細胞ベースの測定法が含まれていた可能性が高い.
  • DNA損傷に対する反応としてGOLPH3のリン酸化の役割を調査し,ウェスタン・ブロッティングや免疫光学のようなテクニックを潜在的に使用した.
  • DNAダメージ誘発剤とDNA-PKの阻害剤を使用した.

主要な成果:

  • ゲルギ装置の分散を誘発するDNA損傷が観察されました.
  • ゲルギ川を通る膀の輸送は,DNAの損傷により抑制されました.
  • DNA-PK媒介によるGOLPH3のリン酸化が,これらの効果の原因となる主要なメカニズムとして特定されました.

結論:

  • DNA損傷反応経路は,ゴルギ装置の組織と機能に直接影響を与えます.
  • DNA-PKによるGOLPH3のリン酸化は,DNAの完全性とゴルギの調節の間の重要なリンクとして機能します.
  • これらの発見は,DNA修復メカニズムとオルゲネルのホメオスタシスの間の新しい交響を明らかにしています.