アクシン依存型ARF転写因子によるDNA結合特異性の構造的基礎
D Roeland Boer1, Alejandra Freire-Rios2, Willy A M van den Berg2
1Institute for Research in Biomedicine (IRB Barcelona), Baldiri Reixac 10-12, 08028 Barcelona, Spain; Institut de Biologia Molecular de Barcelona (IBMB-CSIC), Baldiri Reixac 10-12, 08028 Barcelona, Spain.
Cell
|February 4, 2014
まとめ
植物オキシン反応因子 (ARF) は,DNA結合ドメインの二分化を使用して,特定の遺伝子調節を達成します. このメカニズムにより,ARFは,異なる間隔の好みを持つDNAを結合することによって,植物の発達を制御することができます.
科学分野:
- 植物生物学 植物生物学
- 分子生物学は分子生物学である.
- 遺伝学 遺伝学とは
背景:
- オキシンは,開発を調節する重要な植物ホルモンである.
- アクシン反応因子 (ARF) は,アクシンに対する反応として遺伝子発現を制御する.
- ARF媒介によるオクシン反応における特異性を生成するメカニズムは十分に理解されていません.
研究 の 目的:
- ARFsによって媒介されるオクシン応答の特異性に対する構造的基礎を明らかにする.
- DNA結合と機能におけるARF二分化の役割を調査する.
主な方法:
- ARF1,ARF5/MONOPTEROS (MP),およびARF1-DNA複合体の高解像度の結晶構造の決定.
- DNA結合ドメインとその同位体化能力の分析.
- ARF5/MPのインビヴォ機能評価.
主要な成果:
- ARFのDNA結合ドメインは二分化ドメインとして機能し,協力的なDNA結合を可能にします.
- モノメア型ARFは,類似した固有DNA結合特異性を有する.
- ARFホモダイマーは,DNA結合部位間の間隔に明確な好みを示しています.
- ARF二酸化は,ARF5/MPの in vivo 機能に極めて重要です.
結論:
- ARFのDNA結合ドメインは,二分化ドメインとして機能する.
- ARF二分子は分子キャリパーとして作用し,ARF特有の距離偏好を持つ複雑なDNA部位を結合します.
- これは,オキシン反応の特異性を説明する原子レベルのモデルを提供します.
- ARFダイメリゼーションの理解は,植物の発達調節に関する洞察を提供します.
関連する概念動画
Cooperative Binding of Transcription Regulators
6.0K
Transcriptional regulators bind to specific cis-regulatory sequences in the DNA to regulate gene transcription. These cis-regulatory sequences are very short, usually less than ten nucleotide pairs in length. The short length means that there is a high probability of the exact same sequence randomly occurring throughout the genome. Since regulators can also bind to groups of similar sequences, this further increases the chances of random binding. Transcriptional regulators form...
6.0K
Cooperative Binding of Transcription Regulators
1.8K
1.8K
Eukaryotic Transcription Activators
10.5K
Transcription activators are proteins that promote the transcription of genes from DNA to RNA. In most cases, these proteins contain two separate domains ‒ a domain that binds to DNA and a domain for activating transcription; however, in some cases, a single domain is responsible for both binding and activation of transcription, as seen in the glucocorticoid receptor and MyoD.
The binding domains are capable of recognizing and interacting with regulatory sequences on the DNA. These...
The binding domains are capable of recognizing and interacting with regulatory sequences on the DNA. These...
10.5K
RNA Polymerase II Accessory Proteins
8.9K
Proteins that regulate transcription can do so either via direct contact with RNA Polymerase or through indirect interactions facilitated by adaptors, mediators, histone-modifying proteins, and nucleosome remodelers. Direct interactions to activate transcription is seen in bacteria as well as in some eukaryotic genes. In these cases, upstream activation sequences are adjacent to the promoters, and the activator proteins interact directly with the transcriptional machinery. For example, in...
8.9K
Cell Signaling in Plants
4.5K
Plant cells communicate to coordinate their cycle of growth, flowering and fruiting, and activities in roots, shoots, and leaves in response to the changing environmental conditions. Plant signaling is distinct from animal signaling. Plants primarily utilize enzyme-linked receptors, whereas the largest class of cell-surface receptors in animals are G-protein coupled receptors (GPCRs). Unlike animals, receptor tyrosine kinases are rare in plants. Instead, plants have a diverse class of...
4.5K
Generation of Straight or Branched Actin Filaments
2.9K
The straight or branched structure formation of actin filaments is controlled by nucleating proteins such as the formins and Arp2/3 complex. Formin-mediated assembly results in straight filaments, whereas Arp2/3 protein complex-mediated assembly results in branched actin filaments.
Arp2/3 Complex
Arp2/3 complex is a seven-subunit complex consisting of two proteins similar to actin- Arp2 and Arp3, and five other subunits that help keep Arp2 and Arp3 inactive. When required, the complex is...
Arp2/3 Complex
Arp2/3 complex is a seven-subunit complex consisting of two proteins similar to actin- Arp2 and Arp3, and five other subunits that help keep Arp2 and Arp3 inactive. When required, the complex is...
2.9K


