補助性関節炎におけるTリンパ球によって認識されるミコバクテリアのエピトープのクローン化
W van Eden1, J E Thole, R van der Zee
1Department of Infectious Diseases and Immunology, Veterinary Faculty, State University of Utrecht, The Netherlands.
Nature
|January 14, 1988
まとめ
研究者は,関節軟骨の自己抗原と交叉反応する特定のMycobacterium tuberculosis抗原エピトープを特定しました. この発見は,リウマチ性関節炎と補助性関節炎の治療に新たな可能性をもたらします.
科学分野:
- 免疫学 免疫学とは
- レウマトロジーの病理学
- 微生物学 微生物学とは
背景:
- 補助性関節炎 (AA) は,慢性炎症性関節疾患のラットモデルである.
- AAの病原化には,Tリンパ球がMycobacterium tuberculosisの抗原に反応する.
- M. tuberculosisと関節軟骨の自己抗原の間のクロス反応性エピトープが関与している.
研究 の 目的:
- AAにおけるT細胞の反応性に関与するM. tuberculosisの特異的エピトープを特徴付ける.
- このエピトープが関節炎の治療標的としての可能性を調査する.
主な方法:
- AAラットからの関節性T細胞系とクローンの分離と特徴付け.
- E. coli,切断されたタンパク質,合成ペプチドで表現された菌根性抗原のスクリーニング.
- 特定されたM. tuberculosis抗原に対するT細胞クローン反応性のテスト.
主要な成果:
- AAT細胞クローンは,Mycobacterium bovis BCG抗原内の特定のエピトープ (アミノ酸180-188) を認識しました.
- このエピトープは,関節軟骨の自己抗原と交叉反応性を共有しています.
- この抗原をネズミに投与すると,AA誘導に対する耐性が生じる.
結論:
- 特定されたM. tuberculosisのエピトープは,補助性関節炎の病原性において重要である.
- このエピトープは,リウマチ性関節炎に対する新しい免疫療法の開発の潜在的なターゲットです.
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