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Updated: May 3, 2026

Circulating MicroRNA Quantification Using DNA-binding Dye Chemistry and Droplet Digital PCR
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Circulating MicroRNA Quantification Using DNA-binding Dye Chemistry and Droplet Digital PCR

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DNA-ゴールドナノ粒子探査器を用いた絶対的および直接的なマイクロRNA定量化

Federica Degliangeli1, Prakash Kshirsagar, Virgilio Brunetti

  • 1Center for Biomolecular Nanotechnologies@UniLe, Istituto Italiano di Tecnologia (IIT) ,Via Barsanti, 73010 Arnesano, Lecce, Italy.

Journal of the American Chemical Society
|February 5, 2014
PubMed
まとめ

この研究では,直接的なマイクロRNA定量化のための新しいDNA-ゴールドナノ粒子探査器を導入しています. この方法により,マイクロRNAの感受性および絶対的な測定が可能になり,臨床バイオマーカーとしての使用が進んでいます.

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DNA Microarrays02:34

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...
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Real Time RT-PCR02:57

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...
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