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Updated: May 3, 2026

NMR-Based Activity Assays for Determining Compound Inhibition, IC50 Values, Artifactual Activity, and Whole-Cell Activity of Nucleoside Ribohydrolases
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NMR-Based Activity Assays for Determining Compound Inhibition, IC50 Values, Artifactual Activity, and Whole-Cell Activity of Nucleoside Ribohydrolases

Published on: June 30, 2019

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リボヌクレアースH1二金属イオン触媒のカルシウム阻害

Edina Rosta1, Wei Yang, Gerhard Hummer

  • 1Laboratory of Chemical Physics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health , Bethesda, Maryland 20892-0520, United States.

Journal of the American Chemical Society
|February 7, 2014
PubMed
まとめ

カルシウムイオンは,マグネシウム依存の触媒を阻害することによって,重要なリン酸加工酵素を阻害する. この研究では,特定の部位でのカルシウム結合が酵素機構を変化させ,触媒活性を低下させ,核酸代謝に影響を及ぼすことを明らかにしました.

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