線維細胞ミトーシス中のpp60c-srcの変異したリン酸化と活性化
I Chackalaparampil1, D Shalloway
1Department of Molecular and Cell Biology, Pennsylvania State University, University Park 16802.
Cell
|March 25, 1988
まとめ
ミトーシス過程で,pp60c-srcは新しいリン酸化を受け,そのキナーゼ活性が4〜7倍に増加します. この修正は,Tyr 416/527とは独立したもので,pp60c-srcがミトーシス特異的なイベントを調節することを示唆しています.
科学分野:
- 細胞生物学 細胞生物学
- 分子生物学は分子生物学である.
- バイオケミストリー バイオケミストリー
背景:
- pp60c-srcは,細胞シグナル伝達に関与する非受容体チロシンキナーゼです.
- ミトーシスには,細胞分裂を制御する複雑な規制メカニズムが含まれています.
研究 の 目的:
- ミトーシス中のpp60c-srcの改変と活性を調べる.
- pp60c-srcがミトーシス特有のイベントに作用するかどうかを判断する.
主な方法:
- c-src.srcを過剰発現するNIH 3T3細胞を使用した.
- ミトーシス中に pp60c-src のリン酸化部位 (スレオニン,セリン) を分析した.
- 評価された in vitro キナーゼ活性とフォスフォペプチド分析.
- 固有のpp60c-srcとpp60v-srcのために電泳性運動阻害剤を使用した.
主要な成果:
- pp60c-srcの半分以上は,ミトーシス中にそのアミノ末端領域のスレオニンとセリンリンリン酸化によって変化する.
- ミトスのpp60c-srcは,インビトロキナーゼ活性が4~7倍に増加している.
- 修正はTyr 416/Tyr 527のリン酸化から独立しています.
- 内在的なpp60c-srcとpp60v-srcは,ミトーシス中に同様の変化を示しています.
- 強化された活動と修正は,細胞分裂の近くで解決します.
結論:
- pp60c-srcはミトーシス特有の調節を受けます.
- ミトーシス中の強化されたpp60c-srcキナーゼ活性が,細胞分裂イベントの調節における役割を示唆しています.
- これらの発見は,pp60c-srcシグナル伝達とミトスの進行との潜在的な関連を強調しています.
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