細胞内インスリンによるRNAとタンパク質合成の刺激

D S Miller¹

¹Laboratory of Cellular and Molecular Pharmacology, National Institute of Environmental Health Sciences, Research Triangle Park, NC 27709.

Science (New York, N.Y.)

|April 22, 1988

まとめ

細胞外および細胞内インスリンは,Xenopus laevisの卵細胞の成長を刺激する. インスリンはインスリンです.

科学分野:

* 細胞生物学について
* エンドクリノロジー
* 分子生物学について

背景:

*インスリンは,細胞代謝と様々な組織の成長を調節する重要なホルモンです.
*雌性生殖細胞である卵細胞は,成熟時に重要な代謝変化を経験します.
* 卵細胞におけるインスリン作用の正確なメカニズムは完全に理解されていません.

研究の目的:

*インスリンがXenopus laevisのIV段階卵細胞のマクロモレキュール合成に及ぼす影響を調査する.
* インスリンが細胞表面受容体を通して作用するのか,細胞内での作用なのかを判断する.
*卵細胞内のインスリン作用の潜在的な細胞内部位を特定する.

主な方法:

* ステージIVのXenopus laevis卵細胞は,インビトロ培養を行った.
*卵子は細胞外インスリンに曝されたか,微量注射された細胞内インスリンを受けた.
* 単離された卵細胞核は,直接的な核インスリン効果をテストするために使用されました.
*RNA,タンパク質,およびグリコゲンの合成を測定した.

主要な成果:

* 細胞外および細胞内インスリンの両方が,卵細胞におけるRNA,タンパク質,およびグリコゲン合成を増加させた.
* 外部インスリンと細胞内インスリンの影響は加算的であり,異なる経路を示しています.
*インスリンを単離した核に直接投与すると,RNA合成が刺激される.

結論:

*インスリンがXenopusの卵細胞に顕著なアナボリック効果を発揮し,RNA,タンパク質,およびグリコゲン合成を促進する.
* 細胞外インスリンと細胞内インスリンの作用を媒介するメカニズムは別にある.
* 卵細胞核は,インスリンホルモンの作用の場所として特定されています.

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