関連する実験動画
Updated: Jul 17, 2026
12:30
Monitoring ER/SR Calcium Release with the Targeted Ca2+ Sensor CatchER+
Published on: May 19, 2017
ネズミのマスト細胞における第2のメッセンジャーによるカルシウム流入の調節
R Penner1, G Matthews, E Neher
1Max-Planck-Institut für Biophysikalische Chemie, Göttingen, FRG.
Nature
|August 11, 1988
まとめ
ネズミのマスト細胞は二相カルシウム増加を示し,新しいcAMP活性化塩化物流がIP3依存カルシウム流入と相互作用して,エクソサイトーシスなどのプロセスで細胞内カルシウムレベルを維持します.
科学分野:
- 細胞生物学 細胞生物学
- 生理学 生理学とは
背景:
- 細胞内カルシウムの二相増加は,刺激後の興奮不能の細胞では一般的です.
- カルシウムの持続的な上昇は,様々な細胞プロセスに不可欠です.
研究 の 目的:
- ネズミの腹腔内マスト細胞における持続的な細胞内カルシウム上昇の背後にあるメカニズムを調査する.
- cAMPで活性化された塩化物流とIP3に依存したカルシウム流入の相互作用を調査する.
主な方法:
- クロライド電流を測定するための電気生理学的記録.
- 細胞内カルシウム濃度を監視するためのカルシウムイメージング.
- カルシウムの放出と流入を誘発するための刺激プロトコル.
主要な成果:
- cAMPとCaが活性化された塩化物流がネズミの腹腔内マスト細胞で特定されました.
- この塩化物流は,IP3に依存したカルシウム流入と相互作用する.
- この相互作用は,IP3誘発による最初の放出後に細胞内カルシウムの上昇を維持する.
結論:
- cAMPで活性化された塩化物流とIP3に依存したカルシウム流入を伴う新しいメカニズムが,マスト細胞内の細胞内カルシウムを維持します.
- この相互作用は,エクソサイトーシスを含む,カルシウムに依存するプロセスを調節するための柔軟なシステムを提供します.
関連する概念動画
01:12What are Second Messengers?
Because many receptor binding ligands are hydrophilic, they do not cross the cell membrane and thus their message must be relayed to a second messenger on the inside. There are several second messenger pathways, each with their own way of relaying information. G-protein coupled receptors can activate both phosphoinositol and cyclic AMP (cAMP) second messenger pathways. The phosphoinositol path is active when the receptor induces phospholipase C to hydrolyze the phospholipid,...
01:12What are Second Messengers?
Because many receptor binding ligands are hydrophilic, they do not cross the cell membrane and thus their message must be relayed to a second messenger on the inside. There are several second messenger pathways, each with their own way of relaying information. G-protein coupled receptors can activate both phosphoinositol and cyclic AMP (cAMP) second messenger pathways. The phosphoinositol path is active when the receptor induces phospholipase C to hydrolyze the phospholipid,...
01:15Amplifying Signals via Second Messengers
Many receptor binding ligands are hydrophilic; they do not cross the cell membrane but bind to cell-surface receptors. Thus, their message must be relayed by second messengers present in the cell cytoplasm. There are several second messenger pathways, each with its own way of relaying information. For example, the G protein-coupled receptors can activate both phosphoinositol and cyclic AMP (cAMP) second messenger pathways. The phosphoinositol pathway is active when the receptor induces...
01:11IP3/DAG Signaling Pathway
Membrane lipids such as phosphatidylinositol (PI) are precursors for several membrane-bound and soluble second messengers. Specific kinases phosphorylate PI and produce phosphorylated inositol phospholipids. One such inositol phospholipids are the phosphatidylinositol-4,5 bisphosphate [PI(4,5)P2], present in the inner half of the lipid bilayer. Upon ligand binding, GPCR stimulates Gq proteins to turn on phospholipase Cꞵ. Activated phospholipase Cꞵ cleaves PI(4,5)P2 and produces two-second...
01:27Feedback Regulation of Calcium Concentration
Calcium is an essential signaling molecule required for various cellular functions. Calcium pumps and ion channels on cell and organellar membranes, such as those on the endoplasmic reticulum (ER), regulate calcium concentrations inside the cell. They remain closed, keeping the cytosolic calcium levels low at a resting state.
Various transmembrane receptors, such as G protein-coupled receptors (GPCRs), elicit a response to extracellular signals by increasing cytosolic calcium. Activated GPCRs...
Various transmembrane receptors, such as G protein-coupled receptors (GPCRs), elicit a response to extracellular signals by increasing cytosolic calcium. Activated GPCRs...
01:16Calmodulin-dependent Signaling
Calmodulin (CaM) is a calcium-binding protein in eukaryotes that controls various calcium-regulated cellular processes. It has four calcium-binding sites that bind calcium to form the calcium-calmodulin ( Ca2+-CaM) complex. GPCR stimulation increases the calcium levels in the cells that bind to CaM and induces a conformational change.
The Ca2+-CaM complex does not have enzymatic activity by itself. Instead, the complex binds downstream target proteins, including membrane proteins or enzymes,...
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