植物ナイトレートトランスポーターの窒素吸収の分子基礎 NRT1.1
Joanne L Parker1, Simon Newstead2
1Department of Biochemistry, University of Oxford, Oxford OX1 3QU, UK.
Nature
|February 28, 2014
まとめ
アラビドプシス・サリアナ NRT1.11.1
科学分野:
- 植物生物学 植物生物学
- 分子および細胞生物学
- バイオケミストリー バイオケミストリー
背景:
- NRT1/PTRファミリーは,真核生物とバクテリアのペプチド吸収を通じて窒素の吸収を促進します.
- 植物では,NRT1/PTRトランスポーターは,窒素,ホルモン,二次代謝産物を輸送するために多様化しています.
- 植物窒素トランスポーター1.1 (NRT1.1) は,窒素レベルに対応して,リン酸化時に低親和度状態と高親和度状態を切り替えます.
研究 の 目的:
- アラビドプシス・サリアナ (Arabidopsis thaliana) の窒素結合と輸送の構造的基礎を解明するNRT1.1.1.
- リン酸化がNRT1.1の活性を調節するメカニズムを理解する.
- リガンド認識におけるNRT1/PTRファミリーの進化的分岐を探求する.
主な方法:
- アラビドプシス・サリアナ (Arabidopsis thaliana) のアポと窒素結合構造を特定するためのX線結晶学 NRT1.1.1.
- ニートレートの相互作用を評価するためのインビトロ結合測定法.
- トランスポートアッセイは,トランスポーターの活動を定量化するためのものです.
主要な成果:
- 結晶構造は,窒素の結合部位を明らかにし,ヒスティジン356.6の重要な役割を強調しています.
- 構造的な柔軟性を高め,それによって輸送率を増加させるため,リン酸化が提案されています.
- 構造的な比較は,NRT1/PTRファミリー内の多様な窒素リガンド認識の進化を明らかにします.
結論:
- ヒスティジン356は,アラビドプシス・タリアナ (Arabidopsis thaliana) NRT1.1.1. の窒素結合に欠かせない.
- リン酸化によって引き起こされる構造の変化は,NRT1.1の輸送親和性と速度を調節する.
- NRT1/PTRファミリーは,進化したリガンド特異性とともに,保存された栄養素輸送メカニズムを示しています.
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