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転写スイッチは,マラリア寄生虫の性的発達へのコミットメントの基礎となっている

Björn F C Kafsack¹, Núria Rovira-Graells², Taane G Clark³

¹1] Lewis-Sigler Institute for Integrative Genomics, Princeton University, Princeton, New Jersey 08544, USA [2] Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, Washington 98109, USA (B.F.C.K.); Department of Molecular Biology and Center for Infectious Disease Dynamics, The Pennsylvania State University, State College, Pennsylvania 16802, USA (V.M.C., M.L.).

|February 28, 2014

まとめ

マラリア寄生虫 (プラズモディウム種) PfAP2-Gタンパク質を介して性発育にコミットし,無性からゲメトサイト段階への分化を制御するマスターレギュレータである. この発見は,マラリア対策戦略の新たなターゲットとなる.

科学分野:

分子生物学は分子生物学である.
寄生虫学とは,寄生虫学である.
遺伝学遺伝学とは

背景:

マラリア寄生虫 (プラズモディウム種) 生命周期には,複雑な宿主移行が伴う.
蚊への感染には,雄性ゲメトサイトと雌性ゲメトサイトの分化が必要です.
この性差分化の分子メカニズムは十分に理解されていない.

研究の目的:

プラズモディアム性差異の分子調節体を特定する.
ゲメトサイト形成におけるPfAP2-Gの役割を調査する.
性的なコミットメントの転写制御を解明するために.

主な方法:

PfAP2-G発現とゲメトサイト形成の相関分析.
フォワードとリバースの遺伝学により,PfAP2-Gの機能を評価します.
PfAP2-G結合部位と転写変化の全ゲノム分析.

主要な成果:

PfAP2-Gの発現レベルは,ゲメトサイト形成と強く相関しています.
PfAP2-Gは寄生虫の性差別化に不可欠である.
PfAP2-Gは,重要な早期ゲメトサイト遺伝子を直接調節する.
pfap2-gの自発的な表遺伝子活性化は,ベースラインのゲメトサイト生成を誘発する可能性があります.

結論:

PfAP2-Gは,マラリアの寄生虫における性発達の主な転写調節体として作用する.
この研究は,原生類寄生虫の分化決定を制御する最初の転写スイッチを特定した.
PfAP2-Gの規制を理解することは,新しいマラリアの伝染を阻止する戦略を開発するために不可欠です.

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