関連する実験動画
Updated: Jun 1, 2026
09:14
Static Adhesion Assay for the Study of Integrin Activation in T Lymphocytes
Published on: June 13, 2014
リンパ節ホーミングに関与するリンパ球分子は,軟骨結合タンパク質家族の一員です
I Stamenkovic1, M Amiot, J M Pesando
1Department of Pathology, Harvard Medical School, Massachusetts General Hospital, Boston 02114.
Cell
|March 24, 1989
まとめ
研究者はCDw44抗原の2つの形態,リンパ状および上皮状の形態を特定し,リンパ球の粘着に不可欠である. この発見は,CDw44を明確にしています.
科学分野:
- 免疫学 免疫学とは
- 分子生物学は分子生物学である.
- 細胞生物学 細胞生物学
背景:
- モノクローナル抗体のヘルメスファミリーは,内皮への粘着に関与するリンパ球構造を標的とする.
- この構造は,ネズミのMel-14リンパ節ホーミング受容体のヒトの ekvivalentであると考えられています.
- 以前の研究では,ヘルメス抗原,PGP-1抗原,CDw44抗原に対する抗体は同じ構造を認識することが示されました.
研究 の 目的:
- CDw44抗原をコードするcDNAクローンを分離し,特徴づけるために.
- 異なるCDw44形態の発現パターンを調査する.
- CDw44配列を既知のホーミング受容体と比較する.
主な方法:
- COS細胞でCDw44をコードするcDNAクローンの分離と発現.
- 血液形成細胞および上皮組織におけるCDw44発現の分析.
- CDw44とMel-14抗原および軟骨結合タンパク質の配列比較.
主要な成果:
- CDw44の2つの異なる形が特定されました:リンパ性形と上皮性形です.
- リンパ状の形態は血液形成細胞で表現され,上皮状の形態は上皮質で発見され,がんでは高度に表現されます.
- CDw44は軟骨結合タンパク質と同質性を共有しているが,構造的にはMel-14抗原とは無関係である.
結論:
- CDw44は,異なる発現パターンを持つ少なくとも2つの異なる分子形態に存在する.
- リンパ状の形態は,リンパ細胞の帰着と粘着に役割を果たしている可能性が高い.
- 最初の示唆にもかかわらず,CDw44は,Mel-14リンパ節ホーミング受容体のヒトホモログではありません.
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