関連する実験動画

Updated: May 1, 2026

Quantitation and Analysis of the Formation of HO-Endonuclease Stimulated Chromosomal Translocations by Single-Strand Annealing in Saccharomyces cerevisiae

Quantitation and Analysis of the Formation of HO-Endonuclease Stimulated Chromosomal Translocations by Single-Strand Annealing in Saccharomyces cerevisiae

Published on: September 23, 2011

AddABヘリカーゼ-ニュクレアゼによる転位と, χサイトでその拘束のための構造的基礎

Wojciech W Krajewski¹, Xin Fu², Martin Wilkinson²

¹1] Division of Structural Biology, Institute of Cancer Research, Chester Beatty Laboratories, 237 Fulham Road, London SW3 6JB, UK [2] CRT Discovery Laboratories, Department of Biological Sciences, Birkbeck, University of London, London WC1E 7HX, UK.

|March 28, 2014

まとめ

細菌のDNA修復は,二重鎖の断裂を処理するためにChi配列を認識するAddAB/RecBCD酵素に依存しています. 構造的研究は,Chi結合が酵素活性を停止し,DNAの修復と再結合を導く方法を明らかにしています.

科学分野:

分子生物学は分子生物学である.
バイオケミストリーバイオケミストリー
構造生物学構造生物学とは

背景:

バクテリアの同質再結合は,二重鎖DNAの断裂を修復する.
このプロセスは,Chi配列によって調節されるAddAB/RecBCDヘリカーゼ核酸によって媒介されます.
Chiの認識は,ReCAのロードを開始し,その後のDNA修復に不可欠です.

研究の目的:

AddAB酵素機能の構造的メカニズムを解明する.
Chi 配列が AddAB 活動をどのように調節するかを理解するために.
DNA修復と遺伝子再結合経路に関する洞察を提供するためです.

主な方法:

バチルス・サブティリス AddAB.X線結晶学
Chiを含むDNA基板とATP類似体による複合形成である.
機能的メカニズムを推論するための構造的比較.

主要な成果:

Chi DNAに結合したAddABの結晶構造が得られた.
AddABによるDNA転位と解のためのメカニズムが提案されました.
AddABのChi配列への特定の結合とChi誘発の転位停止が説明されました.

結論:

AddAB/RecBCD酵素の活性がChi配列によって正確に調節されている.
構造的な洞察は,Chiの認識とDNA処理を停止する役割を説明します.
この規則は,DNA修復と遺伝子再結合の信頼性にとって根本的なものです.

さらに関連する動画

Determination of the Optimal Chromosomal Locations for a DNA Element in Escherichia coli Using a Novel Transposon-mediated Approach

Determination of the Optimal Chromosomal Locations for a DNA Element in Escherichia coli Using a Novel Transposon-mediated Approach

Published on: September 11, 2017

Atomic Force Microscopy Investigations of DNA Lesion Recognition in Nucleotide Excision Repair

Atomic Force Microscopy Investigations of DNA Lesion Recognition in Nucleotide Excision Repair

Published on: May 24, 2017

関連する概念動画

Homologous Recombination

Homologous Recombination

The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...

DNA Damage can Stall the Cell Cycle

DNA Damage can Stall the Cell Cycle

In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...

DNA Damage Can Stall the Cell Cycle

DNA Damage Can Stall the Cell Cycle

In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...

Restarting Stalled Replication Forks

Restarting Stalled Replication Forks

DNA replication is initiated at sites containing predefined DNA sequences known as origins of replication. DNA is unwound at these sites by the minichromosome maintenance (MCM) helicase and other factors such as Cdc45 and the associated GINS complex.The unwound single strands are protected by replication protein A (RPA) until DNA polymerase starts synthesizing DNA at the 5’ end of the strand in the same direction as the replication fork. To prevent the replication fork from falling apart,...

Mismatch Repair

Mismatch Repair

Organisms are capable of detecting and fixing nucleotide mismatches that occur during DNA replication. This sophisticated process requires identifying the new strand and replacing the erroneous bases with correct nucleotides. Mismatch repair is coordinated by many proteins in both prokaryotes and eukaryotes.
The Mutator Protein Family Plays a Key Role in DNA Mismatch Repair
The human genome has more than 3 billion base pairs of DNA per cell. Prior to cell division, that vast amount of genetic...

Mechanism of Conjugation

Mechanism of Conjugation

Bacterial conjugation is a mechanism of horizontal gene transfer that enables the exchange of genetic material between bacterial cells through direct contact. This process is facilitated by a donor cell carrying a conjugative plasmid, which encodes genes necessary for pilus formation, DNA replication, and transfer. The conjugative plasmid plays a central role in initiating and executing the transfer of genetic material.The tra region of the conjugative plasmid encodes proteins responsible for...