トランスクリプション制御要素としての"イニシアター"
1Whitehead Institute for Biomedical Research, Cambridge, Massachusetts 02142.
Cell
|April 7, 1989
まとめ
研究者らは,遺伝子転写の開始を正確に制御する新しいイニシアター (Inr) 要素を特定しました. このシンプルで,TATA-boxが欠けているプロモーター要素は,リンパ球における正確な遺伝子発現に不可欠です.
科学分野:
- 分子生物学は分子生物学である.
- 遺伝子規制 遺伝子規制
- バイオケミストリー バイオケミストリー
背景:
- リンパ球特異の末端デオキシヌクレオチジルトランスフェラーゼ遺伝子は,単一のヌクレオチドで転写を開始します.
- TATAボックスがないプロモーターは,転写開始メカニズムを理解する上で課題を提示します.
研究 の 目的:
- 末端デオキシヌクレオチチルトランスフェラーゼ遺伝子の正確な転写イニシアチブに責任を負う要素を特定し,特徴づけること.
- TATA-box-deficient遺伝子における新しいプロモーター要素の役割を調査する.
主な方法:
- インビトロとインビボの転写アッセイ.
- 特定されたプロモーター要素のサイト指向型変異.
- TATA ボックスやSV40 21 bp リピートなどの他のプロモーター要素の存在下での転写開始の分析.
主要な成果:
- トランスクリプション開始部位を含む,17bpのイニシアター (Inr) 要素が特定され,正確な基礎トランスクリプションに十分であった.
- Inrモチーフの突然変異は,転写開始の効率と精度の両方を変化させた.
- Inr 要素は,TATA ボックスまたはSV40 21 bp リピートが存在する場合でも,正確なトランスクリプションの開始を指示し,トランスクリプションレベルを向上させました.
結論:
- イニシアター (Inr) エレメントは,これまでに特定された最も単純な機能プロモーターを表します.
- Inr 要素は,TATA-box-lacking プロモーターが正確な転写イニシアチブを指示するためのメカニズムを提供します.
- この発見は,遺伝子プロモーター構造と規制メカニズムの多様性を理解するのに役立ちます.
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